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Immunology Adaptive Immunity T Cells Cytotoxic Cells

Anti-Nonspecific Cytotoxic Cells抗体[5C.6] (ab2778)

  • Datasheet
Submit a review Q&A (2)References (1)

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Key features and details

  • Mouse monoclonal [5C.6] to Nonspecific Cytotoxic Cells
  • Suitable for: Inhibition Assay, ELISA, IP, WB, Flow Cyt
  • Reacts with: Rat, Horse, Cow, Dog, Pig, Tilapia, Catfish
  • Isotype: IgM

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概述

  • 产品名称

    Anti-Nonspecific Cytotoxic Cells抗体[5C.6]
  • 描述

    小鼠单克隆抗体[5C.6] to Nonspecific Cytotoxic Cells
  • 宿主

    Mouse
  • 经测试应用

    适用于: Inhibition Assay, ELISA, IP, WB, Flow Cytmore details
  • 种属反应性

    与反应: Rat, Horse, Cow, Dog, Pig, Tilapia, Catfish
  • 免疫原

    Tissue, cells or virus corresponding to Nonspecific Cytotoxic Cells. Purified NCC cells from catfish.

  • 阳性对照

    • This antibody as been successfully used in cytotoxic inhibition studies with NCC/NK/LAK cells, panning, and ELISA procedures. In cytotoxicity assays, this antibody is used under saturating conditions to inhibit target cell conjugation.
  • 常规说明

    U.S. Patent Numbers: 5,028,424 and 5,229,494.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • 纯度

    Purified IgM
  • Primary antibody说明

    Non-specific cytotoxic cells (NCCs) in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. One such property is that NCC/natural killer (NK)/lymphokine activated killer (LAK) cells express spontaneous, non-major histocompatibility complex restricted cytotoxic activity. NCC and LAK lyse a variety of transformed murine and human B-cell, T-cell and myeloid targets. A 32 kDa membrane protein [non-specific cytotoxic cell receptor protein (NCCRP-1)] expressed by NCC and certain mammalian NK/LAK cells mediates this cytotoxicity. NCCRP-1 is evolutionarily conserved and is found in species ranging from marine and freshwater teleosts to higher mammals.
  • 克隆

    单克隆
  • 克隆编号

    5C.6
  • 同种型

    IgM
  • 研究领域

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Cytotoxic Cells

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    • Goat Anti-Mouse IgM H&L (ab9167)
    • Rabbit Anti-Mouse IgM mu chain (ab9175)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2778于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Inhibition Assay
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

By Western blot, this antibody detects a 32 kDa protein representing nonspecific cytotoxic cell receptor protein.

Flow Cyt
Use at an assay dependent concentration.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

说明
Inhibition Assay
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

By Western blot, this antibody detects a 32 kDa protein representing nonspecific cytotoxic cell receptor protein.

Flow Cyt
Use at an assay dependent concentration.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

实验方案

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (1)

发表研究结果有使用 ab2778?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2778 被引用在 1 文献中.

  • Jaso-Friedmann L  et al. NCCRP-1: a novel receptor protein sequenced from teleost nonspecific cytotoxic cells. Mol Immunol 34:955-65 (1997). PubMed: 9464530

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

using horse peripheral lymphocytes sees no signal used 1 ug Ab for 10^6 cells in 100 uL used controls to show that flow cytometry is working What concentration and buffer was successfully tested?

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Abcam community

Verified customer

Asked on Sep 15 2011

Answer

Thank you for contacting us. I received the following information from the lab: We don’t have the specific protocol used to test this antibody in flow cytometry. This product was developed outside our lab, and upon checking our files, I was not able to find the specific protocol used to test this product in this application. ab2778 is a mouse IgM. If you are using an anti-Mouse IgG that is fluroescently labeled you would not be able to detect the IgM and thus see no signal. We suggest using an IgM isotype control and an anti-Mouse IgM fluorescently labeled secondary antibody to obtain flow signal. Please also consider that this product should be stored at – 20 deg C. It is shipped with a regular “wet” ice pack but should be frozen upon receipt. Upon first opening the vial the researcher should aliquot it so that each subsequent aliquot undergoes just one more freeze/thaw cycle, as these can damage protein. In addition, I would suggest titration the antibody (i.e. increasing the concentration), but the anti-IgM secondary might be the reason for obtaining no results. I hope this information is of some help to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

回复于 Sep 15 2011

Question

Hi, I want ues your products ab 2778 and ab37131 in ELISA method. Please inform me how can i prepare a standard curve for the concerned proteins to quantify them? Do you also have the standard proteins concerned with the above antibodies? Please let me know at your earliest.

Read More

Abcam community

Verified customer

Asked on Feb 14 2008

Answer

I have been informed by the suppliers for ab37131 and ab2778 that we do not supply the standard proteins unfortunately. For ab2778, anti-Nonspecific Cytotoxic Cell antibody, the lab team has informed me that this antibody was actually tested in ELISA by a customer. The only information we have available is that the researcher used a peptide capture ELISA to determine the active site for the receptor protein NCCRP-1. The lab has suggested that if one wants to quantify NCCRP-1, recombinant peptides would have to be used. Additionally, please keep in mind that this antibody is an IgM isotype. If you experience problems with this product please do contact us, as we have a guarantee on the quality of the antibody in Rat, Catfish, Cow, Dog, Horse, Pig, and Tilapia in ELISA. For ab37131, as I mentioned we don´t have a Rainbow trout CYP1A protein standards. However, I was able to find the specific protocol to be used for ELISA, which I have added to our datasheet and also copied below for your convenience. BUFFERS/REAGENTS 1. Coating buffer:50 mM carbonate-bicarbonate, pH 9.6. 2. PBS:Phosphate buffered saline, pH 7.3 3. Washing buffer: PBS, 0.05% Tween-20 4. Blocking/Dilution buffer: 1% BSA in PBS. 5. Substrate solution. I. ASSAY PROCEDURE Day 1: Coating with samples and positive control Frozen samples and positive control should be thawed on ice. 1. Dilute samples in Coating buffer: Please note: The optimum coating concentration must be determined for each assay, but these dilutions/concentrations can be generally recommended: Liver microsomes: 10 µg/ml Post-mitochondrial supernatants (PMS): 40 µg/ml Plasma samples: 1:1000 Purified proteins: 5-10 µg/ml 2. Dilute the positive control in coating buffer to reach desired concentration. 3. Add 100 µl coating buffer to each of two wells (duplicate analysis) or three wells (triplicate analysis). These wells will be used to determine the Non- Specific Background signal (NSB). 4. Add 100 µl diluted positive control to each of two or three wells. 5. For each sample, add 100 µl to each of two or three wells. 6. Incubate at 4 °C overnight Please note: Coating for 1 hour at room temperature or 37ºC is possible, but this may alter the sensitivity of the assay depending on the nature of the sample. Day 2: Blocking the wells 7. Wash the wells 3 times with 300 µl Washing buffer per well. 8. Add 200 µl of the Blocking/Dilution buffer to each well. Incubate at room temperature for 30-60 min. Incubation with Primary antibody 9. Empty the wells. 10. Dilute the Primary antibody in Blocking/Dilution buffer. Add 100 µl of the antibody solution to each well. Incubate at 37 °C for 1 hour. Please note: Incubation with the Primary antibody may be performed for 2 hours at 37 °C or at 4°C overnight. Depending on the Primary antibody, this may alter the sensitivity of the assay. Incubation with Secondary antibody 11. Wash the wells 3 times with 300 µl Washing buffer per well. 12. Dilute the Secondary antibody in Blocking/Dilution buffer for each plate run in the assay. Add 100 µl of the antibody solution to each well. Incubate at room temperature for 1 hour. Please note: Increasing the incubation time of the Secondary antibody to 2 hours or incubating at 37ºC may increase assay sensitivity. Development of the plate Please note: The Substrate solution should be prepared just before proceeding to the next step. 13. Wash 5 times with 300 µl Washing buffer per well. 14. Add 100 µl substrate solution to each well. Incubate at room temperature for 15 min Please note: If the reaction is weak, the incubation time may be extended up to 30 min. 15. Stop the reaction by adding 50 µl 2M H2SO4 to each well. 16. Read the absorbance at 492 nm. I hope this information will help you, good luck with your research.

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Abcam Scientific Support

回复于 Feb 14 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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