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Anti-NOL3抗体(ab126238)

Submit a review Q&A (1)References (2)
Western blot - Anti-NOL3 antibody (ab126238)
  • Immunocytochemistry/ Immunofluorescence - Anti-NOL3 antibody (ab126238)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody (ab126238)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody (ab126238)

Key features and details

  • Rabbit polyclonal to NOL3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-NOL3抗体
    参阅全部 NOL3 一抗

  • 描述

    兔多克隆抗体to NOL3

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse

  • 免疫原

    Recombinant fragment corresponding to Human NOL3 aa 1-197. (AAH12798)

  • 阳性对照

    • WB: HeLa lysate ICC/IF: HeLa cells IHC-P: HeLa xenograft; muscle tissue

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab126238于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IHC-P
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.
说明
WB
1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IHC-P
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.

靶标

  • 功能

    Isoform 1 may be involved in RNA splicing.
    Isoform 2 may inhibit apoptosis.

  • 组织特异性

    Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.

  • 序列相似性

    Contains 1 CARD domain.

  • 细胞定位

    Cytoplasm and Nucleus > nucleolus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Apoptosis repressor with CARD antibody
      • ARC antibody
      • Muscle enriched cytoplasmic protein antibody
      • Muscle-enriched cytoplasmic protein antibody
      • MYC antibody
      • MYP antibody
      • Nol3 antibody
      • NOL3_HUMAN antibody
      • NOP antibody
      • Nop30 antibody
      • Nucleolar protein 3 (apoptosis repressor with CARD domain) antibody
      • Nucleolar protein 3 antibody
      • Nucleolar protein of 30 kDa antibody
      see all

    图片

    • Western blot - Anti-NOL3 antibody (ab126238)
      Western blot - Anti-NOL3 antibody (ab126238)
      Anti-NOL3 antibody (ab126238) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

      Predicted band size: 24 kDa



      12% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-NOL3 antibody (ab126238)
      Immunocytochemistry/ Immunofluorescence - Anti-NOL3 antibody (ab126238)

      Confocal immunofluorescence analysis of ab126238, at a 1/500 dilution, staining NOL3 in paraformaldehyde fixed HeLa cells (Green). Alpha-tubulin filaments were labeled with another antibody (Red) at a 1/2000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody (ab126238)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody (ab126238)

      ab126238, at a 1/500 dilution, staining NOL3 in paraffin embedded muscle tissue by Immunohistochemistry.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody (ab126238)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody (ab126238)

      ab126238, at 1/500 dilution, staining Human NOL3 in paraffin embedded HeLa xenograft by Immunohistochemistry.

    文献 (2)

    发表研究结果有使用 ab126238？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab126238 被引用在 2 文献中.

    • Xu T  et al. ARC regulates programmed necrosis and myocardial ischemia/reperfusion injury through the inhibition of mPTP opening. Redox Biol 20:414-426 (2019). PubMed: 30415165
    • Li J  et al. SNX13 reduction mediates heart failure through degradative sorting of apoptosis repressor with caspase recruitment domain. Nat Commun 5:5177 (2014). PubMed: 25295779

    客户评价及客户问答

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    Question

    To whom it may concern,



    I am wondering if I use your anti-Apoptosis repressor with CARD antibodies (ab126238 or ab137816) for immunocytochemistry.

    I see your staining results and would like to know,



    1) how did you fix/perbialize for these Abs?

    2) Which is more suitable to detect human ARC in immunocytochemistry?



    Thank you.

    Read More
    Answer

    Thank you very much for contacting us with your enquiry, and I'm very sorry for the delay getting back to you.

    I've received the fixation and permeabilization protocol from the lab:

    1.Add 500µl of 4% paraformaldehyde/PBS into each well, leave for 15mins at room temperature
    2.Remove the paraformaldehyde and wash coverslips by PBS for 3mins, twice.
    3.Add 500µl of 0.1% Triton X-100 in PBS for 4 mins.
    4.Wash cells by PBS for 3mins, twice.

    Both ab126238 and ab137816 work well in ICC with human cells, and they are both fully guaranteed to work in this assay. We don't have much feedback on these antibodies, and they haven't been tested side-by-side. Based on the data, it's difficult to say which is better. They have slightly different specificities (ab126238 for amino acids 1-197, and ab137816 for amino acids 138-192), other than that they are very similar. I am sorry that I don't have a particular suggestion.

    I hope that this information will be useful, but if you have further questions please let me know and I'll be happy to help.

    Read More

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