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重组RabMAb

重组Anti-NOL10抗体[EPR14073-9] (ab181161)

Submit a review Submit a question References (4)
Immunocytochemistry/ Immunofluorescence - Anti-NOL10 antibody [EPR14073-9] (ab181161)
  • Western blot - Anti-NOL10 antibody [EPR14073-9] (ab181161)
  • Anti-NOL10 antibody [EPR14073-9] (ab181161)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14073-9] to NOL10
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-NOL10抗体[EPR14073-9]
    参阅全部 NOL10 一抗

  • 描述

    兔单克隆抗体[EPR14073-9] to NOL10

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC/IFmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Hela, K562, 293T and Huvec cell lysates, Hela cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 纯度

    Tissue culture supernatant

  • 克隆

    单克隆

  • 克隆编号

    EPR14073-9

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab181161于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/10000 - 1/50000. Predicted molecular weight: 80 kDa.
ICC/IF
1/50 - 1/100.
说明
WB
1/10000 - 1/50000. Predicted molecular weight: 80 kDa.
ICC/IF
1/50 - 1/100.

靶标

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-NOL10 antibody [EPR14073-9] (ab181161)
    Immunocytochemistry/ Immunofluorescence - Anti-NOL10 antibody [EPR14073-9] (ab181161)

    Immunocytochemical analysis of Hela cells fixed in acetone at -20℃, labeling NOL10 using ab181161 at 1/100 dilution and Goat anti rabbit IgG (Alexa Fluor® 488) 1/200 dilution (green) and counterstained with DAPI (blue)

  • Western blot - Anti-NOL10 antibody [EPR14073-9] (ab181161)
    Western blot - Anti-NOL10 antibody [EPR14073-9] (ab181161)
    All lanes : Anti-NOL10 antibody [EPR14073-9] (ab181161) at 1/50000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Huvec cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 80 kDa

  • Anti-NOL10 antibody [EPR14073-9] (ab181161)
    Anti-NOL10 antibody [EPR14073-9] (ab181161)

文献 (4)

发表研究结果有使用 ab181161？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab181161 被引用在 4 文献中.

  • Jin X  et al. PQBP5/NOL10 maintains and anchors the nucleolus under physiological and osmotic stress conditions. Nat Commun 14:9 (2023). PubMed: 36599853
  • Iwasaki M  et al. Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells. iScience 25:104289 (2022). PubMed: 35573189
  • Hu X  et al. Identification and Validation of Novel Biomarkers for Diagnosis and Prognosis of Hepatocellular Carcinoma. Front Oncol 10:541479 (2020). PubMed: 33102213
  • Bammert L  et al. Human AATF/Che-1 forms a nucleolar protein complex with NGDN and NOL10 required for 40S ribosomal subunit synthesis. Nucleic Acids Res 44:9803-9820 (2016). PubMed: 27599843

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