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AB181160

重组Anti-NOL10抗体[EPR14073-8]

Anti-NOL10 antibody [EPR14073-8]

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(1 Publication)

Rabbit Recombinant Monoclonal NOL10 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

查看别名

Nucleolar protein 10, NOL10

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-NOL10 antibody [EPR14073-8] (AB181160)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL10 antibody [EPR14073-8] (AB181160)

Immunofluorescent analysis of acetone-fixed HeLa cells labeling NOL10 with ab181160 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution. Counter stained with DAPI.

Western blot - Anti-NOL10 antibody [EPR14073-8] (AB181160)
  • WB

Supplier Data

Western blot - Anti-NOL10 antibody [EPR14073-8] (AB181160)

All lanes:

Western blot - Anti-NOL10 antibody [EPR14073-8] (ab181160) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

293T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 80 kDa

Observed band size: 80 kDa

false

不同偶联物与剂型 (1)

  • Carrier free

    Anti-NOL10 antibody [EPR14073-8] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR14073-8

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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替代产品

Primary Antibodies

AB181161

Anti-NOL10 antibody [EPR14073-9]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NOL10 antibody [EPR14073-9] (AB181161)

  • 0
  • 0
Primary Antibodies

AB235529

Anti-NOL10 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL10 antibody (AB235529)

  • 0
  • 0

搭配使用产品

Primary Antibodies

AB166630

Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)] - Nucleolar Marker

RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)] - Nucleolar Marker (AB166630)

  • 5
  • 2
Primary Antibodies

AB252819

Anti-BOP1 antibody [6H11]

Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-BOP1 antibody [6H11] (AB252819)

  • 0
  • 0
Primary Antibodies

AB22595

Anti-Calnexin antibody - ER Marker

Lab Essentials|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - ER Marker (AB22595)

  • 5
  • 28
Primary Antibodies

AB186735

Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735)

  • 5
  • 16
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯度
Tissue culture supernatant
存储溶液
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Conditional Ambient
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NOL10 also known as nucleolar protein 10 functions primarily in the nucleolus of the cell playing roles in ribosome biogenesis. NOL10 weighs approximately 73 kDa and is present in different human tissues most expressed in spleen and bone marrow. This protein participates in important cellular processes by interacting with ribosomal RNA precursors during ribosome assembly. Its positioning in the nucleolus hints at a focus on ribosome production.
Biological function summary

NOL10 contributes to ribosomal RNA synthesis and processing. It associates with ribosomal RNA processing complexes ensuring mature ribosomal RNA formation. NOL10 works closely with other nucleolar proteins forming part of a larger pre-ribosomal assembly essential for protein synthesis. By doing this NOL10 indirectly supports translation and cell growth.

Pathways

NOL10 is involved in the ribosome biogenesis and RNA processing pathways. It connects with pathways such as the ribosome synthesis pathway interacting with proteins like fibrillarin to promote production of functional ribosomes. It ensures efficient processing within these pathways linking the initial steps of ribosome creation to later stages of protein synthesis.

NOL10's role in ribosome biogenesis links it to conditions like dyskeratosis congenita and certain cancers. Abnormal expression or mutations in NOL10 can result in disrupted ribosomal function contributing to disease progression. It has a connection with proteins such as dyskerin in dyskeratosis congenita highlighting its role in maintaining normal cellular and tissue function.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information NOL10
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 11:69 PubMed40825935

2025

A phosphorylation switch in the Mediator MED15 controls cellular senescence and cognitive decline.

Applications

Unspecified application

Species

Unspecified reactive species

Haozheng Li,Yuanming Zheng,Chunlei Yuan,Jiayi Wang,Xiaying Zhao,Ming Yang,Defei Xiong,Yenan Yang,Yunpeng Dai,Yiming Gao,Yuqi Wang,Lei Xue,Gang Wang
View all publications
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Abcam Product Promise

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