Anti-NOD1抗体(ab105338)
Key features and details
- Rabbit polyclonal to NOD1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-NOD1抗体
参阅全部 NOD1 一抗 -
描述
兔多克隆抗体to NOD1 -
宿主
Rabbit -
特异性
ab105338 is predicted to not cross-react with other CARD family members. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Human NOD1 (C terminal).
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阳性对照
- EL4 cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab105338于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 108 kDa.
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| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 108 kDa. |
靶标
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功能
Enhances caspase-9-mediated apoptosis. Induces NF-kappa-B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathway activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Recruits NLRP10 to the cell membrane following bacterial infection. -
组织特异性
Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon. -
序列相似性
Contains 1 CARD domain.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
翻译后修饰
Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF34 promotes proteasomal degradation and thereby negatively regulates NOD1 for instance in NF-kappa-B activition. -
细胞定位
Cytoplasm. Cell membrane. Apical cell membrane. Basolateral cell membrane. Detected in the cytoplasm and at the cell membrane. Following bacterial infection, localizes to bacterial entry sites in the cell membrane. Recruited to the basolateral and apical membranes in polarized epithelial cells. - Information by UniProt
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数据库链接
- Entrez Gene: 107607 Mouse
- SwissProt: Q8BHB0 Mouse
- Unigene: 28498 Mouse
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别名
- CARD 4 antibody
- CARD4 antibody
- Caspase recruitment domain 4 antibody
see all
图片
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Western blot - Anti-NOD1 antibody (ab105338)Anti-NOD1 antibody (ab105338) at 1 µg/ml + EL4 cell lysate at 15 µg
Secondary
Anti-rabbit secondary antibody
Predicted band size: 108 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab105338 被引用在 4 文献中.
- Huang FC et al. Beneficial effect of probiotics on Pseudomonas aeruginosa-infected intestinal epithelial cells through inflammatory IL-8 and antimicrobial peptide human beta-defensin-2 modulation. Innate Immun 26:592-600 (2020). PubMed: 32988256
- Zhang C et al. Columbianadin Suppresses Lipopolysaccharide (LPS)-Induced Inflammation and Apoptosis through the NOD1 Pathway. Molecules 24:N/A (2019). PubMed: 30717343
- Layunta E et al. NOD1 downregulates intestinal serotonin transporter and interacts with other pattern recognition receptors. J Cell Physiol 233:4183-4193 (2018). PubMed: 29030987
- Bae J et al. Activation of pattern recognition receptors in brown adipocytes induces inflammation and suppresses uncoupling protein 1 expression and mitochondrial respiration. Am J Physiol Cell Physiol 306:C918-30 (2014). PubMed: 24627558