Anti-NOB1抗体(ab224619)
Key features and details
- Rabbit polyclonal to NOB1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-NOB1抗体
参阅全部 NOB1 一抗 -
描述
兔多克隆抗体to NOB1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cynomolgus monkey, Orangutan
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免疫原
Recombinant fragment corresponding to Human NOB1 aa 100-250.
Database link: Q9ULX3 -
阳性对照
- WB: HeLa whole cell lysate; Mouse brain, kidney and stomach tissue lysates. IHC-P: Human thyroid and placenta tissues. ICC/IF: A549 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purity >95%. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224619于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/50 - 1/200.
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| WB |
1/500 - 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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| IHC-P |
1/20 - 1/200.
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| 说明 |
|---|
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ICC/IF
1/50 - 1/200. |
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WB
1/500 - 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
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IHC-P
1/20 - 1/200. |
靶标
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功能
May play a role in mRNA degradation. -
组织特异性
Detected in liver, lung, placenta, endothelial cells and spleen. -
序列相似性
Belongs to the NOB1 family.
Contains 1 PINc domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 101866049 Cynomolgus monkey
- Entrez Gene: 28987 Human
- Entrez Gene: 67619 Mouse
- Entrez Gene: 100174418 Orangutan
- Omim: 613586 Human
- SwissProt: Q4R537 Cynomolgus monkey
- SwissProt: Q9ULX3 Human
- SwissProt: Q8BW10 Mouse
see all -
别名
- Adenocarcinoma antigen recognized by T lymphocytes 4 antibody
- ART 4 antibody
- ART4 antibody
see all
图片
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Western blot - Anti-NOB1 antibody (ab224619)All lanes : Anti-NOB1 antibody (ab224619) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse stomach tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOB1 antibody (ab224619)Paraffin-embedded human thyroid tissue stained for NOB1 using ab224619 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOB1 antibody (ab224619)Paraffin-embedded human placenta tissue stained for NOB1 using ab224619 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-NOB1 antibody (ab224619)A549 (human lung carcinoma cell line) cells stained for NOB1 (green) using ab224619 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab224619 被引用在 4 文献中.
- Xia S et al. Sublethal heat treatment promotes breast cancer metastasis and its molecular mechanism revealed by quantitative proteomic analysis. Aging (Albany NY) 14:1389-1406 (2022). PubMed: 35150481
- Liu H et al. MicroRNA-744 suppresses cell proliferation and invasion of papillary thyroid cancer by directly targeting NOB1. Mol Med Rep 19:1903-1910 (2019). PubMed: 30628685
- Zhang J et al. LncRNA OIP5-AS1 Promotes the Proliferation of Hemangioma Vascular Endothelial Cells via Regulating miR-195-5p/NOB1 Axis. Front Pharmacol 10:449 (2019). PubMed: 31068824
- Deng Y et al. microRNA-744 is downregulated in glioblastoma and inhibits the aggressive behaviors by directly targeting NOB1. Am J Cancer Res 8:2238-2253 (2018). PubMed: 30555741