Anti-nNOS (neuronal) (phospho S1417)抗体(ab5583)
Key features and details
- Rabbit polyclonal to nNOS (neuronal) (phospho S1417)
- Suitable for: IHC-Fr, ICC, WB, IP, ICC/IF, IHC-FoFr
- Reacts with: Mouse, Rat, Rabbit, Human
- Isotype: IgG
概述
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产品名称
Anti-nNOS (neuronal) (phospho S1417)抗体
参阅全部 nNOS (neuronal) 一抗 -
描述
兔多克隆抗体to nNOS (neuronal) (phospho S1417) -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, ICC, WB, IP, ICC/IF, IHC-FoFrmore details -
种属反应性
与反应: Mouse, Rat, Rabbit, Human
预测可用于: Xenopus laevis, Zebrafish
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免疫原
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阳性对照
- WB: nocozadole-treated SH-SY5Y cells; ICC: SH-SY5Y cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (2) |
Use at an assay dependent concentration.
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| ICC |
Use at an assay dependent concentration.
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| WB | (4) |
1/500 - 1/5000. Predicted molecular weight: 160 kDa.
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| IP |
Use at an assay dependent concentration.
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| ICC/IF |
1/10 - 1/100.
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| IHC-FoFr |
Use at an assay dependent concentration. PubMed: 20881244
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| 说明 |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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ICC
Use at an assay dependent concentration. |
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WB
1/500 - 1/5000. Predicted molecular weight: 160 kDa. |
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IP
Use at an assay dependent concentration. |
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ICC/IF
1/10 - 1/100. |
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IHC-FoFr
Use at an assay dependent concentration. PubMed: 20881244 |
靶标
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功能
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. -
组织特异性
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland. -
序列相似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain. -
结构域
The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14. -
翻译后修饰
Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro). -
细胞定位
Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines. - Information by UniProt
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数据库链接
- Entrez Gene: 4842 Human
- Entrez Gene: 18125 Mouse
- Entrez Gene: 100009243 Rabbit
- Entrez Gene: 24598 Rat
- Entrez Gene: 60658 Zebrafish
- Omim: 163731 Human
- SwissProt: P29475 Human
- SwissProt: Q9Z0J4 Mouse
see all -
别名
- 2310005C01Rik antibody
- BNOS antibody
- Constitutive NOS antibody
see all
图片
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Western blot - Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583)All lanes : Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583) at 1/1000 dilution
Lane 1 : untreated SH-SY5Y cells (Human neuroblastoma cell line from bone marrow)
Lane 2 : SH-SY5Y cells (Human neuroblastoma cell line from bone marrow) treated with 100 ng/mL nocodazole
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated secondary antibody
Developed using the ECL technique.
Predicted band size: 160 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583)Immunocytochemistry/Immunofluorescence analysis of nNOS (neuronal) (phospho S1417) (green) showing staining in the cytoplasm and nucleus of SH-SY5Y cells treated with 100 ng/ml nocodazole (left) and untreated SH-SY5Y cells (right). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5583 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab5583 被引用在 22 文献中.
- Bastian P et al. 2-Methoxyestradiol and Hydrogen Peroxide as Promising Biomarkers in Parkinson's Disease. Mol Neurobiol 61:148-166 (2024). PubMed: 37589832
- Wu Y et al. Metabolite asymmetric dimethylarginine (ADMA) functions as a destabilization enhancer of SOX9 mediated by DDAH1 in osteoarthritis. Sci Adv 9:eade5584 (2023). PubMed: 36753544
- Bastian PE et al. 2-Methoxyestradiol Damages DNA in Glioblastoma Cells by Regulating nNOS and Heat Shock Proteins. Antioxidants (Basel) 11:N/A (2022). PubMed: 36290736
- Ho CY et al. CX3CR1-microglia mediates neuroinflammation and blood pressure regulation in the nucleus tractus solitarii of fructose-induced hypertensive rats. J Neuroinflammation 17:185 (2020). PubMed: 32532282
- Uda M et al. Potential roles of neuronal nitric oxide synthase and the PTEN-induced kinase 1 (PINK1)/Parkin pathway for mitochondrial protein degradation in disuse-induced soleus muscle atrophy in adult rats. PLoS One 15:e0243660 (2020). PubMed: 33296434