Anti-nNOS (neuronal)抗体(ab3511)
Key features and details
- Rabbit polyclonal to nNOS (neuronal)
- Suitable for: IHC-Fr, WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Rabbit, Guinea pig, Cow, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-nNOS (neuronal)抗体
参阅全部 nNOS (neuronal) 一抗 -
描述
兔多克隆抗体to nNOS (neuronal) -
宿主
Rabbit -
特异性
Detects nNOS from bovine, mouse, rabbit and rat tissues. This antibody does not detect endothelial NOS (eNOS) or inducible NOS (iNOS). -
经测试应用
适用于: IHC-Fr, WB, IHC-P, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Rabbit, Guinea pig, Cow, Human -
免疫原
Synthetic peptide corresponding to Rat nNOS (neuronal) aa 700-800.
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阳性对照
- WB: Mouse and rat brain tissue lysate. IHC-P: Mouse skeletal muscle tissue, rat cerebellum tissue. IHC-Fr: Rat brain tissue. ICC/IF: SH-SY5Y cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: 100% Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
1/800.
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| WB |
1/1000.
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| IHC-P |
1/200 - 1/400.
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| ICC/IF |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
IHC-Fr
1/800. |
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WB
1/1000. |
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IHC-P
1/200 - 1/400. |
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ICC/IF
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
靶标
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功能
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. -
组织特异性
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland. -
序列相似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain. -
结构域
The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14. -
翻译后修饰
Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro). -
细胞定位
Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines. - Information by UniProt
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数据库链接
- Entrez Gene: 4842 Human
- Entrez Gene: 18125 Mouse
- Entrez Gene: 100009243 Rabbit
- Entrez Gene: 24598 Rat
- Omim: 163731 Human
- SwissProt: P29475 Human
- SwissProt: Q9Z0J4 Mouse
- SwissProt: O19132 Rabbit
see all -
别名
- 2310005C01Rik antibody
- BNOS antibody
- Constitutive NOS antibody
see all
图片
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Western blot - Anti-nNOS (neuronal) antibody (ab3511)
Western blot analysis of nNOS was performed by loading 40µg of Mouse (Lane 1) and Rat Brain (Lane 2) tissue lysate onto a 4-12% Bis-Tris polyacrylamide gel. Proteins were transferred to a Nitrocellulose membrane. Membranes were probed with a rabbit polyclonal antibody (ab3511) recognizing nNOS at a dilution of 1:1000.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab3511)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skeletal muscle tissue sections labeling nNOS (neuronal) with ab3511 at 1/400 (left). Antigen retrieval was performed using 10M sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA in PBS for 30 minutes at room temperature. A biotin-conjugated secondary antibody was used. Right - negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab3511)Immunohistochemistry was performed on normal biopsies of deparaffinized Rat cerebellum tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a Rabbit Polyclonal Antibody recognizing nNOS (ab3511 ) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunohistochemistry (Frozen sections) - Anti-nNOS (neuronal) antibody (ab3511)ab3511 at 1/800 dilution staining nNOS (neuronal) in rat brain tissue section by immunohistochemistry (Frozen sections).
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Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (ab3511)Immunofluorescence analysis of nNOS was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with nNOS Rabbit Polyclonal Antibody (ab3511) at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin (1/300 dilution). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
实验方案
数据表及文件
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Datasheet download
文献 (5)
ab3511 被引用在 5 文献中.
- Singh S et al. Somatostatin-Mediated Regulation of Retinoic Acid-Induced Differentiation of SH-SY5Y Cells: Neurotransmitters Phenotype Characterization. Biomedicines 10:N/A (2022). PubMed: 35203546
- Gao Y et al. Calcium Receptor and Nitric Oxide Synthase Expression in Circular Muscle of Lower Esophagus from Patients with Achalasia. Chin Med J (Engl) 131:2882-2885 (2018). PubMed: 30511698
- Liang C et al. Electro-acupuncture at ST37 and ST25 induce different effects on colonic motility via the enteric nervous system by affecting excitatory and inhibitory neurons. Neurogastroenterol Motil 30:e13318 (2018). PubMed: 29488287
- Evers J et al. Is there a nitrergic modulation of the rat external anal sphincter? Exp Physiol 98:397-404 (2013). ICC/IF ; Rat . PubMed: 22872659
- Sastre E et al. Opposite effect of mast cell stabilizers ketotifen and tranilast on the vasoconstrictor response to electrical field stimulation in rat mesenteric artery. PLoS One 8:e73232 (2013). WB . PubMed: 23977380
