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AB1376

Anti-nNOS (neuronal)抗体

Anti-nNOS (neuronal) antibody

4

(21 Reviews)

|

(82 Publications)

Anti-nNOS (neuronal) antibody (ab1376) is a goat polyclonal antibody detecting nNOS (neuronal) in Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human.

- Over 80 publications
- Trusted since 2002

查看别名

Nitric oxide synthase 1, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1, N-NOS, nNOS, bNOS, NOS1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (AB1376)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (AB1376)

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2µg/mL).

Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody (AB1376)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody (AB1376)

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab1376 for 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control : Unimmunized goat IgG (black line).

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

Flow Cyt (Intra), ICC/IF

applications

免疫原

Synthetic Peptide within Human Nitric oxide synthase, brain aa 1400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P29475

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "10 µg/mL", "FlowCytIntra-species-notes": "<p></p>" } } }
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您是否考虑以下替代产品？

AB76067

Anti-nNOS (neuronal) antibody [EP1855Y]

5
10 Reviews
查看产品
为什么推荐这个？

为向您提供最高品质的产品，我们在不断升级产品范围。我们选择了这个替代产品供您参考，因为它提供了更好的性能，更容易和更方便的使用，或交期更快。

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产品详情

What is this antibody validated in?
Anti-nNOS (neuronal) antibody (ab1376) is a goat polyclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Trusted by the scientific community
Anti-nNOS (neuronal) (ab1376) was first used in a scientific publication in 2002 and has been cited over 80 times in peer-reviewed journals.

Reviewed by scientists
Anti-nNOS (neuronal) (ab1376) has over 20 independent reviews from customers.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
存储溶液
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NNOS also known as nitric oxide synthase 1 or NOS1 is a critical enzyme in the nervous system. It catalyzes the production of nitric oxide from L-arginine. This enzyme weighing approximately 160 kDa is expressed in neurons and you can find it in regions like the brain and spinal cord. Notably nNOS is part of the NOS family which also includes eNOS and iNOS but its primary function is in neuronal tissues.
Biological function summary

NNOS plays a significant role in neurotransmission and neurovascular regulation. As a part of protein complexes nNOS closely interacts with other signaling molecules to modulate neuronal communication. The production of nitric oxide by nNOS acts as a neurotransmitter influencing brain functions such as synaptic plasticity and memory formation. Additionally due to its role in neuronal activity antibodies like anti-neuronal antibodies can have interactions that can be studied using various assays including the NOS assay.

Pathways

NNOS serves essential roles within the neuronal nitric oxide signaling pathway and the glutamatergic signaling pathway. In these contexts nNOS associates with proteins involved in cell signaling such as calmodulin which regulates its activity. By participating in these pathways nNOS contributes to the regulation of vascular tone and synaptic transmission linking it to neurological processes and supporting neuronal communication.

NNOS shows significant connections to neurological disorders and cardiovascular diseases. In conditions like stroke and neurodegenerative diseases dysregulation of nNOS activity leads to excessive production of nitric oxide which can result in oxidative stress and neuronal damage. Furthermore its relation with eNOS in the cardiovascular system influences vascular function making it a critical player in hypertension studies. Understanding nNOS interactions through diseases highlights the possibility of targeted treatments using neuronal antibodies and anti-neuronal antibodies against specific pathways.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
See full target information Nitric oxide synthase, brain
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文献 (82)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroanatomy 17:1210502 PubMed38020216

2023

Secretagogin as a marker to distinguish between different neuron types in human frontal and temporal cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Tapia-González,Javier DeFelipe

eNeuro 10: PubMed37364998

2023

A Selective Projection from the Subthalamic Nucleus to Parvalbumin-Expressing Interneurons of the Striatum.

Applications

Unspecified application

Species

Unspecified reactive species

Krishnakanth Kondabolu,Natalie M Doig,Olaoluwa Ayeko,Bakhtawer Khan,Alexandra Torres,Daniela Calvigioni,Konstantinos Meletis,Tibor Koós,Peter J Magill

Basic and clinical andrology 33:11 PubMed37198550

2023

Implantation of skin-derived precursor Schwann cells improves erectile function in a bilateral cavernous nerve injury rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolei Ma,Wende Yang,Pan Nie,Zhenbin Zhang,Zehong Chen,Hongbo Wei

eNeuro 10: PubMed36963833

2023

CaMKIIα Promoter-Controlled Circuit Manipulations Target Both Pyramidal Cells and Inhibitory Interneurons in Cortical Networks.

Applications

Unspecified application

Species

Unspecified reactive species

Judit M Veres,Tibor Andrasi,Petra Nagy-Pal,Norbert Hajos

Journal of translational medicine 21:100 PubMed36759871

2023

Renal nerve stimulation identifies renal innervation and optimizes the strategy for renal denervation in canine.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Liu,Yidan Li,Hao Zhou,Weijie Chen,Yanping Xu,Huaan Du,Bo Zhang,Tianli Xia,Dan Li,Zhenhong Ou,Ruotian Tang,Qingsong Chen,Binyi Zhao,Yuehui Yin

The EMBO journal 42:e111348 PubMed36524441

2022

STIM1 and ORAI1 form a novel cold transduction mechanism in sensory and sympathetic neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara J Buijs,Bruno Vilar,Chun-Hsiang Tan,Peter A McNaughton

World journal of gastroenterology 28:3854-3868 PubMed36157548

2022

Involvement of nitrergic neurons in colonic motility in a rat model of ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Rong Li,Yan Li,Yuan Jin,Mang Xu,Hong-Wei Fan,Qian Zhang,Guo-He Tan,Jing Chen,Yun-Qing Li

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao

Anatomical record (Hoboken, N.J. : 2007) 306:2264-2275 PubMed35717590

2022

Neuronal nitric oxide synthase and calbindin expression in sympathetic preganglionic neurons following capsaicin treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina V Porseva,Nikita D Preobrazhensky

Science advances 8:eabn3567 PubMed35687680

2022

Daily changes in light influence mood via inhibitory networks within the thalamic perihabenular nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Tenley Weil,K M Daly,Hector Yarur Castillo,Michael B Thomsen,Huikun Wang,Maria E Mercau,Samer Hattar,Hugo Tejeda,Diego C Fernandez
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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