Anti-NNMT抗体(ab223513)
Key features and details
- Rabbit polyclonal to NNMT
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NNMT抗体
参阅全部 NNMT 一抗 -
描述
兔多克隆抗体to NNMT -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human NNMT aa 1 to the C-terminus.
Database link: P40261 -
阳性对照
- WB: HepG2, HeLa and A549 whole cell lysates. IHC-P: Human liver and kidney tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223513于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
|
|
| IHC-P |
1/20 - 1/200.
|
| 说明 |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
|
IHC-P
1/20 - 1/200. |
靶标
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功能
Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. -
组织特异性
Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas. -
序列相似性
Belongs to the NNMT/PNMT/TEMT family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 4837 Human
- Omim: 600008 Human
- SwissProt: P40261 Human
- Unigene: 503911 Human
- Unigene: 623245 Human
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别名
- EC 2.1.1.1 antibody
- Nicotinamide N methyltransferase antibody
- Nicotinamide N-methyltransferase antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody (ab223513)
Paraffin-embedded human kidney tissue stained for NNMT using ab223513 at 1/100 dilution in immunohistochemical analysis.
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Western blot - Anti-NNMT antibody (ab223513)All lanes : Anti-NNMT antibody (ab223513) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody (ab223513)Paraffin-embedded human liver tissue stained for NNMT using ab223513 at 1/100 dilution in immunohistochemical analysis.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab223513 被引用在 1 文献中.
- Zhang X et al. Ferroptosis is governed by differential regulation of transcription in liver cancer. Redox Biol 24:101211 (2019). PubMed: 31108460