Anti-nNav1.5抗体[4G8:1G7] (ab62388)
![Immunocytochemistry/ Immunofluorescence - Anti-nNav1.5 antibody [4G8:1G7] (ab62388)](https://www.abcam.cn/ps/products/62/ab62388/Images/ab62388-75718-anti-nnav15-antibody-4g81g7-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-nNav1.5 antibody [4G8:1G7] (ab62388)")
Key features and details
- Mouse monoclonal [4G8:1G7] to nNav1.5
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgM
概述
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产品名称
Anti-nNav1.5抗体[4G8:1G7] -
描述
小鼠单克隆抗体[4G8:1G7] to nNav1.5 -
宿主
Mouse -
特异性
The immunogen used was 14 amino acids long and only 6 are specific for the neonatal variant therefore it is possible it may also bind the adult splice variant. -
经测试应用
适用于: ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human nNav1.5.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
单克隆 -
克隆编号
4G8:1G7 -
同种型
IgM -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62388于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use 1-2µg for 106 cells.
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
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| 说明 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use 1-2µg for 106 cells. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
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图片
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Immunocytochemistry/ Immunofluorescence - Anti-nNav1.5 antibody [4G8:1G7] (ab62388)Ab62388 (10ug/ml) staining human Nav1.5 in Nav1.5-HEK293 recombinant cell line by immunocytochemistry.
A: Plasma membrane localisation of Nav1.5 identified by ab62388 detected by anti-mouse Alexa Fluor-488 conjugated antibody.
B: DAP1 co-staining of cell nuclei. -
![Flow Cytometry - Anti-nNav1.5 antibody [4G8:1G7] (ab62388)](https://www.abcam.cn/ps/products/62/ab62388/Images/ab62388-75717-anti-nnav15-antibody-4g81g7-flow-cytometry.jpg)
Flow Cytometry - Anti-nNav1.5 antibody [4G8:1G7] (ab62388)Overlay histogram showing SHSY-5Y cells stained with ab62388 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62388, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879>) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SHSY-5Y cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab62388 被引用在 4 文献中.
- Utrilla RG et al. Kir2.1-Nav1.5 Channel Complexes Are Differently Regulated than Kir2.1 and Nav1.5 Channels Alone. Front Physiol 8:903 (2017). PubMed: 29184507
- Vikram A et al. Sirtuin 1 regulates cardiac electrical activity by deacetylating the cardiac sodium channel. Nat Med 23:361-367 (2017). PubMed: 28191886
- Guo Q et al. A novel mutation in the SCN5A gene contributes to arrhythmogenic characteristics of early repolarization syndrome. Int J Mol Med 37:727-33 (2016). PubMed: 26820605
- Guo L et al. Estimating the risk of drug-induced proarrhythmia using human induced pluripotent stem cell-derived cardiomyocytes. Toxicol Sci 123:281-9 (2011). ICC/IF ; Human . PubMed: 21693436