重组Anti-nmt55 / p54nrb抗体[EPR5270]
Anti-nmt55 / p54nrb antibody [EPR5270]
- RabMAb
- Recombinant
- KO Validated
- 了解详情
Be the first to review this product! Submit a review
|
(10 Publications)
Rabbit Recombinant Monoclonal nmt55 / p54nrb antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 10 publications.
查看别名
NRB54, NONO, Non-POU domain-containing octamer-binding protein, NonO protein, 54 kDa nuclear RNA- and DNA-binding protein, 55 kDa nuclear protein, p54(nrb), p54nrb, NMT55
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-nmt55 / p54nrb antibody [EPR5270] (AB133574)
Immunocytochemistry/Immunofluorescence analysis of Molt-4 (human acute lymphoblastic leukemia) cells labelling nmt55/p54nrb with purified ab133574 at 1/1500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1 : primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody [EPR5270] (AB133574)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labelling nmt55 /p54nrb using unpurified ab133574 at a 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody [EPR5270] (AB133574)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human bladder carcinoma tissue labelling nmt55 / p54nrb using unpurified ab133574 at a 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody [EPR5270] (AB133574)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling nmt55/p54nrb with purified ab133574 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody [EPR5270] (AB133574)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling nmt55/p54nrb with purified ab133574 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody [EPR5270] (AB133574)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling nmt55/p54nrb with purified ab133574 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
- WB
Lab
Western blot - Anti-nmt55 / p54nrb antibody [EPR5270] (AB133574)
Lanes 1-3 : Merged signal (red and green). Green - ab133574 observed at 63 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab133574 Anti-nmt55 / p54nrb antibody [EPR5270] was shown to specifically react with nmt55 / p54nrb in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266244 (knockout cell lysate ab257160) was used. Wild-type and nmt55 / p54nrb knockout samples were subjected to SDS-PAGE. ab133574 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human NONO (nmt55 / p54nrb) knockout HEK-293T cell lysate (<a href='/products/cell-lysates/human-nono-nmt55-p54nrb-knockout-hek-293t-cell-lysate-ab257160'>ab257160</a>) at 20 µg
Lane 3:
MOLT-4 cell lysate at 20 µg
Secondary
Lanes 1 - 3:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>)
Lanes 1 - 3:
Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>)
Predicted band size: 54 kDa
Observed band size: 63 kDa,37 kDa
false
- WB
Unknown
Western blot - Anti-nmt55 / p54nrb antibody [EPR5270] (AB133574)
Lanes 1-3 : Merged signal (red and green). Green - ab133574 observed at 63 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab133574 Anti-nmt55 / p54nrb antibody [EPR5270] was shown to specifically react with nmt55 / p54nrb in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266244 (knockout cell lysate ab257160) was used. Wild-type and nmt55 / p54nrb knockout samples were subjected to SDS-PAGE. ab133574 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
NONO knockout HEK293T cell lysate at 20 µg
Lane 3:
MOLT-4 cell lysate at 20 µg
Predicted band size: 54 kDa
Observed band size: 63 kDa
false
- WB
Unknown
Western blot - Anti-nmt55 / p54nrb antibody [EPR5270] (AB133574)
All lanes:
Western blot - Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574) at 1/1000 dilution
Lane 1:
PC3 cell lysate at 10 µg
Lane 2:
MOLT4 cell lysate at 10 µg
Lane 3:
HeLa cell lysate at 10 µg
Lane 4:
MCF7 cell lysate at 10 µg
Lane 5:
A673 cell lysate at 10 µg
Lane 6:
RAW 264.7 cell lysate at 10 µg
Lane 7:
PC12 cell lysate at 10 µg
Lane 8:
NIH/3T3 cell lysate at 10 µg
Secondary
All lanes:
Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
false
- WB
Unknown
Western blot - Anti-nmt55 / p54nrb antibody [EPR5270] (AB133574)
Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.
All lanes:
Western blot - Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574) at 1/10000 dilution
Lane 1:
Molt-4 cell lysate at 20 µg
Lane 2:
A673 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution
Predicted band size: 54 kDa
false
- WB
Unknown
Western blot - Anti-nmt55 / p54nrb antibody [EPR5270] (AB133574)
Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.
All lanes:
Western blot - Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574) at 1/10000 dilution
All lanes:
RAW264.7 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution
Predicted band size: 54 kDa
false
- WB
Unknown
Western blot - Anti-nmt55 / p54nrb antibody [EPR5270] (AB133574)
Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.
All lanes:
Western blot - Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574) at 1/10000 dilution
All lanes:
PC-12 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution
Predicted band size: 54 kDa
false
- OI-RD Scanning
Unknown
OI-RD Scanning - Anti-nmt55 / p54nrb antibody [EPR5270] (AB133574)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
不同偶联物与剂型 (3)
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-nmt55 / p54nrb antibody [EPR5270]
-
Anti-nmt55 / p54nrb antibody [EPR5270] - BSA and Azide free
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-nmt55 / p54nrb antibody [EPR5270]
反应性数据
产品详情
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
性能和储存信息
形式
纯化工艺
存储溶液
运输条件
推荐的短期储存时间
推荐的短期储存条件
推荐的长期储存条件
分装信息
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
P54nrb is a multifunctional protein critical in nuclear processes. It forms part of a complex involved in RNA processing and nuclear retention of edited RNA. This complex also includes the proteins PSF and PSPC1 contributing to its role in transcription regulation and RNA stability. p54nrb impacts gene expression through its ability to bind to DNA and RNA influencing numerous regulatory pathways within the cell.
Pathways
P54nrb has essential roles in gene expression and RNA maturation pathways. It closely interacts with the RNA polymerase II machinery affecting transcription elongation. The protein has an established connection with the steroid receptor RNA activator (SRA) to modulate transcriptional responses. It is related to other DBHS proteins such as PSF which work together for coordinate regulation of gene expression and splice site selection.
产品实验方案
- Visit the General protocols
- Visit the Troubleshooting
靶点信息
文献 (10)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 16:7954 PubMed40858606
2025
Applications
Unspecified application
Species
Unspecified reactive species
Journal of virology 98:e0029724 PubMed39499073
2024
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in immunology 14:1290185 PubMed38274825
2024
Applications
Unspecified application
Species
Unspecified reactive species
Scientific reports 13:7134 PubMed37130848
2023
Applications
Unspecified application
Species
Unspecified reactive species
Biochemistry and biophysics reports 27:101052 PubMed34179518
2021
Applications
Unspecified application
Species
Unspecified reactive species
Science advances 6:eaaz9072 PubMed32923585
2020
Applications
Unspecified application
Species
Unspecified reactive species
Cell biology international 44:166-176 PubMed31393052
2019
Applications
Unspecified application
Species
Unspecified reactive species
Cell 175:1902-1916.e13 PubMed30550788
2018
Applications
Unspecified application
Species
Unspecified reactive species
Nucleic acids research 46:2204-2217 PubMed29390093
2018
Applications
Unspecified application
Species
Unspecified reactive species
PLoS genetics 13:e1006658 PubMed28273136
2017
Applications
ICC/IF
Species
Human
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com