Anti-nmt55 / p54nrb抗体(ab50411)
Key features and details
- Goat polyclonal to nmt55 / p54nrb
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-nmt55 / p54nrb抗体
参阅全部 nmt55 / p54nrb 一抗 -
描述
山羊多克隆抗体to nmt55 / p54nrb -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Dog, Cynomolgus monkey
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免疫原
Synthetic peptide:
C-NRAAPGAEFAPNK
, corresponding to C terminal amino acids 455-467 of Human nmt55/ p54nrb -
阳性对照
- Mouse Brain lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50411于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
1 hour primary incubation is recommended for this product. |
| IHC-P |
Use a concentration of 3 - 6 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 3 - 6 µg/ml. |
靶标
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功能
DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site (By similarity). Involved in pre-mRNA splicing, probably as an heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. Nono is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. -
组织特异性
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines. -
疾病相关
Note=A chromosomal aberration involving NONO may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;X)(p11.2;q13.1) with TFE3. -
序列相似性
Contains 2 RRM (RNA recognition motif) domains. -
翻译后修饰
The N-terminus is blocked. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4841 Human
- Entrez Gene: 53610 Mouse
- Entrez Gene: 317259 Rat
- Omim: 300084 Human
- SwissProt: Q15233 Human
- SwissProt: Q99K48 Mouse
- SwissProt: Q5FVM4 Rat
- Unigene: 533282 Human
see all -
别名
- 52 kDa subunit antibody
- 54 kDa nuclear RNA and DNA binding protein antibody
- 54 kDa nuclear RNA- and DNA-binding protein antibody
see all
图片
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Western blot - Anti-nmt55 / p54nrb antibody (ab50411)Anti-nmt55 / p54nrb antibody (ab50411) at 0.1 µg/ml + Mouse Brain lysate (35µg protein in RIPA buffer).
Predicted band size: 55 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Western blot - Anti-nmt55 / p54nrb antibody (ab50411)All lanes : Anti-nmt55 / p54nrb antibody (ab50411) at 1/2000 dilution
Lane 1 : MEF whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Donkey anti-goat IgG HRP
Predicted band size: 55 kDa
Observed band size: 55 kDa
This image was submitted courtesy of an Abreview, please refer to Abreview for further experimental details.
数据表及文件
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Datasheet download
文献 (5)
ab50411 被引用在 5 文献中.
- Xu (徐兴丽) X et al. Knockout of the NONO Gene Inhibits Neointima Formation in a Mouse Model of Vascular Injury. Arterioscler Thromb Vasc Biol 41:1428-1445 (2021). PubMed: 33626912
- Bottini S et al. Post-transcriptional gene silencing mediated by microRNAs is controlled by nucleoplasmic Sfpq. Nat Commun 8:1189 (2017). PubMed: 29084942
- Rajesh C et al. The splicing-factor related protein SFPQ/PSF interacts with RAD51D and is necessary for homology-directed repair and sister chromatid cohesion. Nucleic Acids Res 39:132-45 (2011). WB ; Mouse . PubMed: 20813759
- Ong SA et al. Rasd1 Modulates the Coactivator Function of NonO in the Cyclic AMP Pathway. PLoS One 6:e24401 (2011). WB ; Human . PubMed: 21915321
- Rajesh C et al. The interaction profile of homologous recombination repair proteins RAD51C, RAD51D and XRCC2 as determined by proteomic analysis. Proteomics 9:4071-86 (2009). WB ; Mouse . PubMed: 19658102
