Anti-NMT2抗体(ab224045)
Key features and details
- Rabbit polyclonal to NMT2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NMT2抗体
参阅全部 NMT2 一抗 -
描述
兔多克隆抗体to NMT2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Cow
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免疫原
Recombinant fragment corresponding to Human NMT2 aa 50-250.
Database link: O60551 -
阳性对照
- IHC: Human testis tissue. WB: RT4 cell lysate. ICC/IF: U-2 OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224045于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 57 kDa.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 57 kDa. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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功能
Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins. -
序列相似性
Belongs to the NMT family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 282049 Cow
- Entrez Gene: 9397 Human
- Omim: 603801 Human
- SwissProt: Q9N181 Cow
- SwissProt: O60551 Human
- Unigene: 60339 Human
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别名
- EC 2.3.1.97 antibody
- Glycylpeptide N tetradecanoyltransferase 2 antibody
- Glycylpeptide N-tetradecanoyltransferase 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMT2 antibody (ab224045)
Immunohistochemical analysis of human Testis tissue labelling NMT2 using ab121234, shows positive membranous and cytoplasmic staining in cells in semiferous ducts.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMT2 antibody (ab224045)Immunohistochemical analysis of human cerebral cortex tissue labelling NMT2 using ab121234, shows positive cytoplasmic staining in neural cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMT2 antibody (ab224045)Immunohistochemical analysis of human Tonsil tissue labelling NMT2 using ab121234, shows positive membranous staining in non-germinal center cells.
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Western blot - Anti-NMT2 antibody (ab224045)Anti-NMT2 antibody (ab224045) at 1/100 dilution + RT4 (human urinary bladder cancer cell line) cell lysate
Predicted band size: 57 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-NMT2 antibody (ab224045)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for NMT2 (green) using ab224045 at 4 µg/ml in ICC/IF.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab224045 被引用在 1 文献中.
- Zhu G et al. N-Myristoylation by NMT1 Is POTEE-Dependent to Stimulate Liver Tumorigenesis via Differentially Regulating Ubiquitination of Targets. Front Oncol 11:681366 (2021). PubMed: 34136404