Anti-NMNAT3抗体(ab121030)

Thank you for your reply.

I am sorry that this antibody did not perform as stated on the datasheet. As requested, I have asked our Finance department to issue a credit note for you.

Credit note ID: 21735

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Thank you for taking the time to get back to us with this information.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry therenot many further tips to provide on this occasion to help improve the results. Regrettably I can suggest you have received a bad vial on this occasion. In this case, I am pleased to offer you a free of charge replacement or credit note in compensation.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Thank you for getting back to us in regards to the problems encountered when using the Anti-NMNAT3 antibody (ab121030).

xxxx is currently away form the office but I will try my best to assist you in resolving these problems. In order to try and understand the problem further, can I just ask the following:

1. The blot provided shows 3 other targets which have been probed, Nmnat1, Nmnat2 and Nmpt. Could you please let me know if there were all probed on the same membrane with stripping in between each probe or was a fresh membrane used every time?

2. Could you let me know which antibodies were used to probe these other 3 targets (catalogue numbers if you wouldn't mind)?

3. Which secondary antibodies were used with each target?

4. Have any "no primary" controls been performed for these secondary antibodies?

The reason I ask is that although the anti-Nmnat3 antibody ab121030 is definitely showing a strong band of an unexpected size (˜50 kDa), and it is very difficult to determine if there is even a specific band in the expected region (˜28 kDa), this is also the case for some of the other targets probed. It appears that the Nmnat1 and Nmnat2 are also both showing a band at ˜50 kDa, whereas a band at 32 and 37 kDa respectively would be expected. I am just wondering if this non-specificity that is being observed may be down to one of the secondary antibodies non-specifically binding the mouse IgG present in the sample (heavy chain expected at ˜50 kDa). Or if a similar event is contributing to the problem.

I thank you for your continued cooperation and look forward to receiving your reply.

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to reassure you that ab121030 is tested and covered by our 6 month guarantee for use inWB and mouse samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

Reviewing this case, I would like to offer some suggestions to help optimize the results. I would also appreciate if you can confirm some further details:

1. Please confirm the order number and date of purchase. Was this Abcam order ref 1078622 ordered 26th April?

2. Please confirm how old were the samples? How had they been stored? Have fresh samples been tried?

3. I would appreciate if you are able toprovide an image, including molecular weight markers, which will help us to assess the results.

4. I can suggest to try BSA rather than milk to block. Changing blocking agent can sometimes help to improve results.

5. How were the lysate samples prepared? I can recommend to use RIPA lysis buffer and to reduce and denature at 95oC for 5 minutes in sample buffer containing SDS and mercaptoethanol if this has not already been done.

6. I can suggest to trya lower concentration of antibody to help reduce the background if this has not already been tried?

7. Could you confirm if the secondary antibody isworking well with other primary antibodies? What were the results of the no primary control? The concentration of the secondary may need to be reduced in order to help optimize the results.

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.