Anti-Nmnat1/NMNAT抗体[OTI1F7] (ab118270)
![Western blot - Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270)](https://www.abcam.cn/ps/products/118/ab118270/Images/ab118270-130486-anti-nmnat1-antibody-oti1f7-western-blot.jpg "Western blot - Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270)")
Key features and details
- Mouse monoclonal [OTI1F7] to Nmnat1/NMNAT
- Suitable for: WB, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
概述
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产品名称
Anti-Nmnat1/NMNAT抗体[OTI1F7]
参阅全部 Nmnat1/NMNAT 一抗 -
描述
小鼠单克隆抗体[OTI1F7] to Nmnat1/NMNAT -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human Nmnat1/NMNAT aa 1 to the C-terminus. Produced in HEK-293T cells (NP_073624).
Database link: Q9HAN9 -
阳性对照
- WB: Nmnat1/NMNAT transfected HEK-293T cell lysate. ICC/IF: COS-7 cells transiently transfected with Nmnat1/NMNAT.
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常规说明
Clone OTI1F7 (formerly 1F7).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography. -
克隆
单克隆 -
克隆编号
OTI1F7 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab118270于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 31 kDa.
|
|
| ICC/IF |
1/100.
|
| 说明 |
|---|
|
WB
1/2000. Predicted molecular weight: 31 kDa. |
|
ICC/IF
1/100. |
靶标
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功能
Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP (PubMed:17402747). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency (PubMed:17402747). Can use triazofurin monophosphate (TrMP) as substrate (PubMed:17402747). Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+) (PubMed:17402747). For the pyrophosphorolytic activity, prefers NAD(+) and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively (PubMed:17402747). Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257). Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+) (PubMed:17402747). Protects against axonal degeneration following mechanical or toxic insults. -
组织特异性
Widely expressed with highest levels in skeletal muscle, heart and kidney. Also expressed in the liver pancreas and placenta. Widely expressed throughout the brain. -
通路
Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.
Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1. -
疾病相关
Leber congenital amaurosis 9 -
序列相似性
Belongs to the eukaryotic NMN adenylyltransferase family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 64802 Human
- Omim: 608700 Human
- SwissProt: Q9HAN9 Human
- Unigene: 633762 Human
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别名
- EC 2.7.7.1 antibody
- LCA9 antibody
- Leber's congenital amaurosis 9 antibody
see all
图片
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Western blot - Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270)All lanes : Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY Nmnat1/NMNAT cDNA transfected HEK-293T cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 31 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270)](https://www.abcam.cn/ps/products/118/ab118270/Images/ab118270-130485-anti-nmnat1-antibody-oti1f7-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270)pCMV6-ENTRY Nmnat1/NMNAT cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for Nmnat1/NMNAT using ab118270 at a 1/100 dilution in ICC/IF.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab118270 尚未被引用在任何文献中。