重组Anti-NME2抗体[EPR8351] (ab131329)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8351] to NME2
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Chinese hamster
Related conjugates and formulations
概述
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产品名称
Anti-NME2抗体[EPR8351]
参阅全部 NME2 一抗 -
描述
兔单克隆抗体[EPR8351] to NME2 -
宿主
Rabbit -
特异性
The immunogen used to raise this antibody shares 85% homology with human NME1. Cross-reactivity with this protein has not been confirmed experimentally.
The mouse, rat and Chinese hamster recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and Chinese hamster.
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经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human, Chinese hamster -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: LnCaP, PC-12, CHO, 293T, Human spleen and Human thyroid lysates. Rat and mouse kidney tissue lysates; IHC: Human kidney tissue and human cerebellum tissue; Flow Cyt (intra): HEK293 cells; ICC/IF: CHO-S cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解离常数(KD)
KD = 3.35 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8351 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131329于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/20.
For unpurified use at 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000. Predicted molecular weight: 17 kDa.
For unpurified use at 1/1000 - 1/10000. |
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IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. The mouse, rat and Chinese hamster recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and Chinese hamster. |
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ICC/IF |
Use at an assay dependent concentration.
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说明 |
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Flow Cyt (Intra)
1/20. For unpurified use at 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Predicted molecular weight: 17 kDa. For unpurified use at 1/1000 - 1/10000. |
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. The mouse, rat and Chinese hamster recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and Chinese hamster. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the NDK family. -
细胞定位
Cytoplasm. Nucleus. Isoform 2 is mainly cytoplasmic and isoform 1 and isoform 2 are excluded from the nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 4831 Human
- Entrez Gene: 654364 Human
- Entrez Gene: 18103 Mouse
- Entrez Gene: 83782 Rat
- Omim: 156491 Human
- SwissProt: P22392 Human
- SwissProt: Q01768 Mouse
- SwissProt: P19804 Rat
see all -
别名
- C myc purine binding transcription factor PUF antibody
- C myc transcription factor antibody
- C-myc purine-binding transcription factor PUF antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [EPR8351] (ab131329)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling NME2 with purified ab131329 at 1/500 dilution (0.22 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-NME2 antibody [EPR8351] (ab131329) at 1/1000 dilution (purified)
Lane 1 : Mouse kidney lysate
Lane 2 : Rat kidney lysate
Lane 3 : CHO-S (Chinese hamster ovary epithelial cell) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa -
Immunocytochemistry/ Immunofluorescence analysis of CHO-S (Chinese hamster ovary epithelial cell) cells labeling NME2 with purified ab131329 at 1/50 dilution (2.18 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of HEK-293 (Human embryonickidney epithelial cell) cells labeling NME2 with purified ab131329 at 1/20 dilution (5µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-NME2 antibody [EPR8351] (ab131329) at 1/1000 dilution (purified)
Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa -
All lanes : Anti-NME2 antibody [EPR8351] (ab131329) at 1/1000 dilution (unpurified)
Lane 1 : LnCaP cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : CHO cell lysate
Lane 4 : 293T cell lysate
Lane 5 : Human spleen lysate
Lane 6 : Human thyroid lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [EPR8351] (ab131329)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling NME2 with unpurified ab131329 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab131329 被引用在 3 文献中.
- Adam K et al. The Potential Functional Roles of NME1 Histidine Kinase Activity in Neuroblastoma Pathogenesis. Int J Mol Sci 21:N/A (2020). PubMed: 32392889
- Qiu J et al. Cellular nucleic acid binding protein suppresses tumor cell metastasis and induces tumor cell death by downregulating heterogeneous ribonucleoprotein K in fibrosarcoma cells. Biochim Biophys Acta 1840:2244-52 (2014). Human . PubMed: 24594223
- Chen S et al. Mechanistic studies for the role of cellular nucleic-acid-binding protein (CNBP) in regulation of c-myc transcription. Biochim Biophys Acta 1830:4769-77 (2013). WB, IP ; Human . PubMed: 23774591