Anti-NMDAR2B (phospho S1480)抗体(ab73014)
Key features and details
- Rabbit polyclonal to NMDAR2B (phospho S1480)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
概述
-
产品名称
Anti-NMDAR2B (phospho S1480)抗体
参阅全部 NMDAR2B 一抗 -
描述
兔多克隆抗体to NMDAR2B (phospho S1480) -
宿主
Rabbit -
特异性
ab73014 is specific for ~180k NMDAR2B protein phosphorylated at Ser1480. Immunolabeling of the NMDAR2B band is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. Immunolabeling is also blocked by lambda-phosphatase treatment. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat
预测可用于: Mouse, Chicken, Cow, Dog, Human, Non human primates, Zebrafish
-
免疫原
Synthetic peptide corresponding to Rat NMDAR2B (phospho S1480).
Database link: Q00960 -
阳性对照
- Rat hippocampal lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.50
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab73014 is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab73014于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa).
|
| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa). |
靶标
-
功能
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
组织特异性
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
细胞定位
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 417965 Chicken
- Entrez Gene: 537804 Cow
- Entrez Gene: 494009 Dog
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Entrez Gene: 100334883 Zebrafish
- Omim: 138252 Human
see all -
别名
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
图片
-
Western blot - Anti-NMDAR2B (phospho S1480) antibody (ab73014)All lanes : Anti-NMDAR2B (phospho S1480) antibody (ab73014) at 1/1000 dilution
Lane 1 : Rat hippocampal lysate
Lane 2 : Rat hippocampal lysate incubated in lambda-phosphatase (1200 units for 30 min)
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 85 kDa. We are unsure as to the identity of these extra bands.
The phosphospecificity of the labeling is shown in the second lane (lambda-phosphatase). The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab73014 被引用在 5 文献中.
- Sun Z et al. Casein kinase 2 attenuates brain injury induced by intracerebral hemorrhage via regulation of NR2B phosphorylation. Front Cell Neurosci 16:911973 (2022). PubMed: 35928572
- Lie E et al. SALM4 negatively regulates NMDA receptor function and fear memory consolidation. Commun Biol 4:1138 (2021). PubMed: 34588597
- Zhang C et al. Protopanaxadiol ginsenoside Rd protects against NMDA receptor-mediated excitotoxicity by attenuating calcineurin-regulated DAPK1 activity. Sci Rep 10:8078 (2020). PubMed: 32415270
- McKay S et al. The Developmental Shift of NMDA Receptor Composition Proceeds Independently of GluN2 Subunit-Specific GluN2 C-Terminal Sequences. Cell Rep 25:841-851.e4 (2018). PubMed: 30355491
- Emanuele M et al. Exogenous Alpha-Synuclein Alters Pre- and Post-Synaptic Activity by Fragmenting Lipid Rafts. EBioMedicine 7:191-204 (2016). PubMed: 27322472