重组Anti-NMDAR2B (phospho S1303)抗体[EP1858Y] (ab81271)
![Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.cn/ps/products/81/ab81271/Images/ab81271-296742-anti-nmdar2b-phospho-s1303-antibody-ep1858y-western-blot.jpg "Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1858Y] to NMDAR2B (phospho S1303)
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-NMDAR2B (phospho S1303)抗体[EP1858Y]
参阅全部 NMDAR2B 一抗 -
描述
兔单克隆抗体[EP1858Y] to NMDAR2B (phospho S1303) -
宿主
Rabbit -
经测试应用
适用于: Dot blot, WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human, mouse and rat brain tissue lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1858Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81271于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot |
1/1000.
|
|
| WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 166 kDa.
|
| 说明 |
|---|
|
Dot blot
1/1000. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 166 kDa. |
应用说明
Is unsuitable for IHC-P or IP.
靶标
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功能
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
组织特异性
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
细胞定位
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q13224 Human
- SwissProt: Q01097 Mouse
- SwissProt: Q00960 Rat
- Unigene: 654430 Human
see all -
别名
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
图片
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Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat brain tissue lysate. The membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.cn/ps/products/81/ab81271/Images/ab81271-296741-anti-nmdar2b-phospho-s1303-antibody-ep1858y-western-blot.jpg)
Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human brain tissue lysate. The membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
-
![Dot Blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.cn/ps/products/81/ab81271/Images/ab81271-296735-anti-nmdar2b-phospho-s1303-antibody-ep1858y-dot-blot.jpg)
Dot Blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)Dot blot analysis of NMDAR2B (phospho S1303) phospho peptide (Lane 1) and NMDAR2B non-phospho peptide (Lane 2) labelling NMDAR2B (phospho S1303) with ab81271 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.cn/ps/products/81/ab81271/Images/ab81271-96453-anti-nmdar2b-phospho-s1303-antibody-ep1858y-western-blot.jpg)
Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution
Lane 1 : Mouse brain lysates (untreated)
Lane 2 : Mouse brain lysates (treated with AP)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP labelled IgG at 1/2000 dilution
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted? -
![Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.cn/ps/products/81/ab81271/Images/ab81271-5-benefits-of-recombinant-antibodies.png)
Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (31)
ab81271 被引用在 31 文献中.
- Luo M et al. Gestational dexamethasone exposure impacts hippocampal excitatory synaptic transmission and learning and memory function with transgenerational effects. Acta Pharm Sin B 13:3708-3727 (2023). PubMed: 37719378
- Choi JE et al. Round-window delivery of lithium chloride regenerates cochlear synapses damaged by noise-induced excitotoxic trauma via inhibition of the NMDA receptor in the rat. PLoS One 18:e0284626 (2023). PubMed: 37216352
- Pitzer EM et al. Developmental deltamethrin: Sex-specific hippocampal effects in Sprague Dawley rats. Curr Res Toxicol 3:100093 (2022). PubMed: 36393872
- Wang XS et al. Activation of GIPR Exerts Analgesic and Anxiolytic-Like Effects in the Anterior Cingulate Cortex of Mice. Front Endocrinol (Lausanne) 13:887238 (2022). PubMed: 35712239
- Sun Z et al. Casein kinase 2 attenuates brain injury induced by intracerebral hemorrhage via regulation of NR2B phosphorylation. Front Cell Neurosci 16:911973 (2022). PubMed: 35928572