AB183942

重组Anti-NMDAR2B抗体[EPR19295]

Anti-NMDAR2B antibody [EPR19295]

20ul selling size
RabMAb
Recombinant
了解详情

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(12 Publications)

Rabbit Recombinant Monoclonal NMDAR2B antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 12 publications.

查看别名

NMDAR2B, GRIN2B, GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3, hNR3

2 Images

Supplier Data

Western blot - Anti-NMDAR2B antibody [EPR19295] (AB183942)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1,2,3,4,5,6 and 7 : 30 seconds; Lane 8 and 9 : 8 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID : 9252405; PMID : 15470155).

All lanes:

Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942) at 1/1000 dilution

Lane 1:

Human hippocampus lysate at 20 µg

Lane 2:

Human fetal liver lysate at 20 µg

Lane 3:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg

Lane 4:

Mouse liver lysate at 20 µg

Lane 5:

Mouse kidney lysate at 20 µg

Lane 6:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 7:

Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg

Lane 8:

Mouse brain lysate at 20 µg

Lane 9:

Mouse hippocampus lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 166 kDa

Observed band size: 166 kDa

false

Supplier Data

Western blot - Anti-NMDAR2B antibody [EPR19295] (AB183942)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 9252405; PMID : 15470155).

All lanes:

Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942) at 1/5000 dilution

Lane 1:

Rat brain lysate at 20 µg

Lane 2:

Rat hippocampus lysate at 20 µg

Lane 3:

Rat liver lysate at 20 µg

Lane 4:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 166 kDa

Observed band size: 166 kDa

false

Exposure time: 8s

不同偶联物与剂型 (1)

Carrier free

Anti-NMDAR2B antibody [EPR19295] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19295

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NMDAR2B also known as NR2B or GluN2B functions as a subunit of the NMDA receptor complex. It plays a role in synaptic transmission and plasticity in the central nervous system. The target weighs approximately 166 kDa. It is highly expressed in the cerebral cortex hippocampus and striatum. NMDAR2B interacts with other subunits in the NMDA receptor which assemble to form a functional ion channel that allows for calcium ion influx when activated by glutamate and glycine.

Biological function summary

The NMDAR2B subunit contributes to the regulation of synaptic strength and is essential for processes involved in learning and memory. As part of the NMDA receptor complex it mediates excitatory neurotransmission and is involved in synaptic plasticity processes such as long-term potentiation (LTP). These functions are significant for cognitive function and neural development. The receptor's role in signal transduction is aided by the unique properties conferred by the NMDAR2B subunit such as its high affinity for glycine and slower deactivation kinetics.

Pathways

NMDAR2B is involved in the glutamatergic signaling pathway which is important for neural communication. It also participates in the calcium signaling pathway affecting cellular responses to external stimuli. The protein interacts with CaMKII and PSD-95 which are proteins that influence synaptic strength and architecture through these pathways. Its involvement links it to a variety of signaling events important for brain function.

Alterations in NMDAR2B have been associated with neurodegenerative conditions such as Alzheimer's disease and neurodevelopmental disorders like schizophrenia. The protein's malfunction can lead to abnormal synaptic connectivity and excitotoxicity. It is linked to other proteins associated with these diseases such as beta-amyloid in Alzheimer's and dopamine receptor dysregulation in schizophrenia indicating its role in disease progression and symptom manifestation.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed : 24272827, PubMed : 24863970, PubMed : 26875626, PubMed : 26919761, PubMed : 27839871, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). Participates in synaptic plasticity for learning and memory formation by contributing to the long-term depression (LTD) of hippocampus membrane currents (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed : 24272827, PubMed : 24863970, PubMed : 26875626, PubMed : 26919761, PubMed : 27839871, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed : 26875626, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity).

See full target information GRIN2B

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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 11:e15673 PubMed37078449

2023

Altered expression of synaptic proteins and adhesion molecules in the hippocampus and cortex following the onset of diabetes in nonobese diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Takumi Yokokawa,Kohei Kido,Koji Sato,Tatsuya Hayashi,Satoshi Fujita

iScience 25:104754 PubMed35938049

2022

The endoplasmic reticulum membrane complex promotes proteostasis of GABA receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Angela L Whittsette,Ya-Juan Wang,Ting-Wei Mu

The European journal of neuroscience 55:18-31 PubMed34902209

2021

Hippocampal AMPA- and NMDA-induced cGMP signals are mainly generated by NO-GC2 and are under tight control by PDEs 1 and 2.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Giesen,Evanthia Mergia,Doris Koesling,Michael Russwurm

Frontiers in aging neuroscience 13:772980 PubMed34916926

2021

Deficits in N-Methyl-D-Aspartate Receptor Function and Synaptic Plasticity in Hippocampal CA1 in APP/PS1 Mouse Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Le Xu,Yiying Zhou,Linbo Hu,Hongde Jiang,Yibei Dong,Haowei Shen,Zhongze Lou,Siyu Yang,Yunxin Ji,Liemin Ruan,Xiaoqin Zhang

International journal of molecular sciences 22: PubMed34830164

2021

Nitric Oxide-Dependent Mechanisms Underlying MK-801- or Scopolamine-Induced Memory Dysfunction in Animals: Mechanistic Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Paulina Cieślik,Anna Siekierzycka,Adrianna Radulska,Agata Płoska,Grzegorz Burnat,Piotr Brański,Leszek Kalinowski,Joanna M Wierońska

Frontiers in neuroscience 15:641284 PubMed33994921

2021

Valproic Acid Induces Autism-Like Synaptic and Behavioral Deficits by Disrupting Histone Acetylation of Prefrontal Cortex ALDH1A1 in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Liu,Mei Tan,Boli Cheng,Si Wang,Lu Xiao,Jiang Zhu,Qionghui Wu,Xi Lai,Qian Zhang,Jie Chen,Tingyu Li

Oxidative medicine and cellular longevity 2021:6634181 PubMed33833851

2021

Low-Dose Copper Exposure Exacerbates Depression-Like Behavior in ApoE4 Transgenic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Xu,Kaiwu He,Kaiqin Zhang,Chao Yang,Lulin Nie,Ding Dan,Jianjun Liu,Chang-E Zhang,Xifei Yang

Pharmacological research 160:105082 PubMed32679183

2020

Butyrate, but not propionate, reverses maternal diet-induced neurocognitive deficits in offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Linchao Yu,Xin Zhong,Yu He,Yuan Shi

Neuron 105:506-521.e7 PubMed31813652

2019

Usp9X Controls Ankyrin-Repeat Domain Protein Homeostasis during Dendritic Spine Development.

Applications

Unspecified application

Species

Unspecified reactive species

Sehyoun Yoon,Euan Parnell,Maria Kasherman,Marc P Forrest,Kristoffer Myczek,Susitha Premarathne,Michelle C Sanchez Vega,Michael Piper,Thomas H J Burne,Lachlan A Jolly,Stephen A Wood,Peter Penzes

Journal of Alzheimer's disease : JAD 73:517-528 PubMed31796678

2019

Carnosic Acid Reverses the Inhibition of ApoE4 on Cell Surface Level of ApoER2 and Reelin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Maoxiao Feng,Donghai Cui,Yi Li,Jian Shi,Lan Xiang,Hong Bian,Zhiyong Ma,Wen Xia,Guangwei Wei

View all publications

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