重组Anti-NMDAR2A (phospho Y1325)抗体[EPR27295-51] (ab302930)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR27295-51] to NMDAR2A (phospho Y1325)
- Suitable for: WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
概述
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产品名称
Anti-NMDAR2A (phospho Y1325)抗体[EPR27295-51]
参阅全部 NMDAR2A 一抗 -
描述
兔单克隆抗体[EPR27295-51] to NMDAR2A (phospho Y1325) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IHC-Fr,IHC-P or IP -
种属反应性
与反应: Mouse, Rat
不与反应: Human -
免疫原
This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. -
阳性对照
- WB: Mouse brain tissue lysate (untreated membrane), rat hippocampus tissue lysate (untreated membrane), rat cerebral cortex tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR27295-51 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab302930于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 165 kDa).
|
说明 |
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WB
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 165 kDa). |
靶标
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功能
NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits. -
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily. -
细胞定位
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 14811 Mouse
- Entrez Gene: 24409 Rat
- SwissProt: P35436 Mouse
- SwissProt: Q00959 Rat
- Unigene: 2953 Mouse
- Unigene: 9710 Rat
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别名
- EPND antibody
- FESD antibody
- GluN2A antibody
see all
图片
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All lanes : Anti-NMDAR2A (phospho Y1325) antibody [EPR27295-51] (ab302930) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate 20 µg (untreated membrane)
Lane 2 : Mouse brain tissue lysate 20 µg (phosphatase treated membrane)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under non-reducing conditions.
Predicted band size: 165 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Lysates were freshly made and used immediately to minimize protein degradation.
-
All lanes : Anti-NMDAR2A (phospho Y1325) antibody [EPR27295-51] (ab302930) at 1/1000 dilution
Lane 1 : Rat hippocampus tissue lysate 20 µg (untreated membrane)
Lane 2 : Rat hippocampus tissue lysate 20 µg (phosphatase treated membrane)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Performed under non-reducing conditions.
Predicted band size: 165 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Lysates were freshly made and used immediately to minimize protein degradation.
-
Anti-NMDAR2A (phospho Y1325) antibody [EPR27295-51] (ab302930) at 1/1000 dilution + Rat cerebral cortex tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Performed under non-reducing conditions.
Predicted band size: 165 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Lysates were freshly made and used immediately to minimize protein degradation.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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