Anti-NMDAR2A (phospho Y1325)抗体(ab106590)
Key features and details
- Rabbit polyclonal to NMDAR2A (phospho Y1325)
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
概述
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产品名称
Anti-NMDAR2A (phospho Y1325)抗体
参阅全部 NMDAR2A 一抗 -
描述
兔多克隆抗体to NMDAR2A (phospho Y1325) -
宿主
Rabbit -
特异性
Specific for the ~180k NMDAR2A protein phosphorylated at Tyr1325 in Western blots. Immunolabelling is completely blocked by preadsorption with the phosphopeptide but not by the corresponding dephosphopeptide. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat
预测可用于: Cow, Dog
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免疫原
Synthetic peptide corresponding to NMDAR2A (C terminal).
Database link: Q12879 -
阳性对照
- Rat hippocampal lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.50
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106590于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000. Predicted molecular weight: 165 kDa.
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| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 165 kDa. |
靶标
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功能
NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits. -
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily. -
细胞定位
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 524212 Cow
- Entrez Gene: 490012 Dog
- Entrez Gene: 14811 Mouse
- Entrez Gene: 24409 Rat
- SwissProt: P35436 Mouse
- SwissProt: Q00959 Rat
- Unigene: 2953 Mouse
- Unigene: 9710 Rat
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别名
- EPND antibody
- FESD antibody
- GluN2A antibody
see all
图片
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Western blot - Anti-NMDAR2A (phospho Y1325) antibody (ab106590)All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab106590) at 1/1000 dilution
Lane 1 : Rat hippocampal lysate
Lane 2 : Rat hippocampal lysate with preadsorption with the phospho-peptide
Predicted band size: 165 kDa
Observed band size: ~180 kDa why is the actual band size different from the predicted? -
Western blot - Anti-NMDAR2A (phospho Y1325) antibody (ab106590)This image is courtesy of an Abreview submitted by Dr Xiaofan WangAll lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab106590) at 1/500 dilution
All lanes : Rat brain whole tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP Goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 165 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab106590 被引用在 4 文献中.
- Loera-Valencia R et al. High levels of 27-hydroxycholesterol results in synaptic plasticity alterations in the hippocampus. Sci Rep 11:3736 (2021). PubMed: 33580102
- Wang Y et al. GluN2A/ERK/CREB Signaling Pathway Involved in Electroacupuncture Regulating Hypothalamic-Pituitary-Adrenal Axis Hyperactivity. Front Neurosci 15:703044 (2021). PubMed: 34658758
- Go BS et al. Glutamatergic neurotransmission in the prefrontal cortex mediates the suppressive effect of intra-prelimbic cortical infusion of BDNF on cocaine-seeking. Eur Neuropsychopharmacol 26:1989-1999 (2016). PubMed: 27765467
- Taniguchi S et al. Involvement of NMDAR2A tyrosine phosphorylation in depression-related behaviour. EMBO J 28:3717-29 (2009). PubMed: 19834457