重组Anti-NMDAR2A抗体[EPR19694] (ab183939)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19694] to NMDAR2A
- Suitable for: WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
概述
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产品名称
Anti-NMDAR2A抗体[EPR19694]
参阅全部 NMDAR2A 一抗 -
描述
兔单克隆抗体[EPR19694] to NMDAR2A -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse and rat hippocampus and brain lysates; His-tagged mouse NMDAR2A recombinant protein fragment (aa aa22-326).
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19694 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183939于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Detects a band of approximately 165 kDa (predicted molecular weight: 165 kDa).
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说明 |
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WB
1/1000. Detects a band of approximately 165 kDa (predicted molecular weight: 165 kDa). |
靶标
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功能
NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits. -
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily. -
细胞定位
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 14811 Mouse
- Entrez Gene: 24409 Rat
- SwissProt: P35436 Mouse
- SwissProt: Q00959 Rat
- Unigene: 2953 Mouse
- Unigene: 9710 Rat
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别名
- EPND antibody
- FESD antibody
- GluN2A antibody
see all
图片
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All lanes : Anti-NMDAR2A antibody [EPR19694] (ab183939) at 1/1000 dilution
Lane 1 : Mouse hippocampus lysate
Lane 2 : Rat hippocampus lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lane 5 : Mouse heart lysate
Lane 6 : Rat kidney lysate
Lane 7 : Mouse spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 165 kDa
Observed band size: 165 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 3 minutes; Lane 2: 30 seconds; Lane 3, 4, 5, 6 and 7: 3 minutes.
NMDAR2A has been previously documented to be specifically expressed in neuron cells. (PMID: 12127670).
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All lanes : Anti-NMDAR2A antibody [EPR19694] (ab183939) at 1/5000 dilution
Lane 1 : His-tagged mouse NMDAR2A recombinant protein fragment (aa aa22-326)
Lane 2 : His-tagged mouse NMDAR2B recombinant protein fragment (aa27-322)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 165 kDa
Observed band size: 165 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 1 seconds; Lane 2: 3 minutes.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab183939 被引用在 1 文献中.
- Giesen J et al. Hippocampal AMPA- and NMDA-induced cGMP signals are mainly generated by NO-GC2 and are under tight control by PDEs 1 and 2. Eur J Neurosci 55:18-31 (2022). PubMed: 34902209