Anti-NMDAR1抗体(ab52177)

Thanks for your email.

I have located your order. I am happy to send a vial of anti-GFAP antibody ab53554 as the replacement. We do have this product in stock and I will ship it Monday for Tuesday delivery. I will send you an email containing the order reference number for this replacement order today, for your records.

Also, I'm afraid our order processing system is not designed to handle your request to combine this replacement order with an upcoming order from your lab.

I hope this is helpful. Please contact me again if you have any further questions.

Thanks for your email.

I did see the western blot image that you kindly attached to your last email. There are a few bands found in the "No Primary Ab" blot but not nearly as many as can be seen in the blot on the left which includes the primary. In the blot on the left it is clear that many non specific bands are being detected by the primary antibody. There is no way to determine which band is NMDAR1 based on the data you have provided.

This problem does sometimes occur with antibodies. It is for cases like this that we have a guarantee which essentially states that if the antibody is not performing as expected based on the online antibody datasheet, we are happy to provide a replacement of any primary antibody, credit or refund, as long as the customer follows up with us within 6 months of receiving the product.

I believe in this case the vial of antibody you received is not working as expected.

Again, Iam sorry this antibody is giving you trouble. I am happy to offer a replacement of any primary antibody, credit or refund. If you are interested, please let me know how you wish to proceed. In your reply, kindly include the PO number or Abcam order reference number associated with this purchase of ab52177 so I can process your request.

Thanks for your email.

I am sorry this antibody is giving you trouble. I am happy to offer a replacement of any primary antibody, credit or refund. If you are interested, please let me know how you wish to proceed. In your reply, kindly include the PO number or Abcam order reference number associated with this purchase of ab52177 so I can process your request.

Thanks for your reply.

For a negative control, I was thinking of samples in which you do not expect to see NMDAR1 expression. Since there is more than one bandinyour sample lanesI think it would be helpful to compare a sample where there is definitely no NMDAR1 expression.

A "no primary" control is a good idea too to determine if any of the bands you are seeing are due to non-specific interactions of the secondary antibody. But if this secondary antibody has been recently used with other primary antibodies with expected results, then this "no primary" control is probably not necessary.

I hope this is helpful. Please contact me again if you have any further questions.

Thanks so much for all your troubleshooting efforts!

Do the gels from your colleague your the gel image you sent on Sunday contain any negative controls samples, ie. samples in which you do not expect to see NMDAR1 expression? If so, please note which lanes are the negative control lanes. Thanks!

Thanks for your enquiry and for including the western blot image.

It does not appear that NMDAR1 is being detectedon your western blot. I just have a couple additional questions:

1. How much protein was loaded per lane on the western blot?

2. What dilutions of the primary were tested?

3. Were any other antibodies used successfully to blot these same tissues?

Thanks in advance for the additional information!