Anti-NMDAR1抗体(ab52177)
Key features and details
- Rabbit polyclonal to NMDAR1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-NMDAR1抗体
参阅全部 NMDAR1 一抗 -
描述
兔多克隆抗体to NMDAR1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide within Human NMDAR1 aa 849-913. The exact sequence is proprietary. From around the phosphorylation site of serine 897 (R-S-S-K-D).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
Without Mg+2 and Ca+2 -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52177于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 105 kDa.
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IHC-P | (1) |
Use at an assay dependent concentration.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 105 kDa. |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. -
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily. -
翻译后修饰
NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. -
细胞定位
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities. - Information by UniProt
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数据库链接
- Entrez Gene: 2902 Human
- Entrez Gene: 14810 Mouse
- Omim: 138249 Human
- SwissProt: Q05586 Human
- SwissProt: P35438 Mouse
- Unigene: 558334 Human
- Unigene: 278672 Mouse
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别名
- GluN1 antibody
- Glutamate [NMDA] receptor subunit zeta-1 antibody
- Glutamate receptor ionotropic N methyl D aspartate 1 antibody
see all
图片
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All lanes : Anti-NMDAR1 antibody (ab52177) at 1/500 dilution
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) whole cell lysate
Lane 2 : Y79 (Human retinoblastoma cell line) whole cell lysate
Lane 3 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 105 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa, 75 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 antibody (ab52177)
ab52177 at 1/50 dilution staining NMDAR1 in human brain tissue by immunohistochemistry (paraffin embedded tissue) in the presence (right image) or absence (left image) of the immunizing peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab52177 被引用在 18 文献中.
- Tang F et al. Munc18-1 Contributes to Hippocampal Injury in Septic Rats Through Regulation of Syntanxin1A and Synaptophysin and Glutamate Levels. Neurochem Res 48:791-803 (2023). PubMed: 36335177
- Chiprés-Tinajero GA et al. Increased immunoreactivity of glutamate receptors, neuronal nuclear protein and glial fibrillary acidic protein in the hippocampus of epileptic rats with fast ripple activity. Exp Brain Res 239:2015-2024 (2021). PubMed: 33909110
- Jiang H et al. Anti-N-Methyl-D-Aspartate Receptor Encephalitis Associated with Ovarian Teratoma in South China-Clinical Features, Treatment, Immunopathology, and Surgical Outcomes of 21 Cases. Dis Markers 2021:9990382 (2021). PubMed: 34093900
- Rivas-Santisteban R et al. N-Methyl-D-aspartate (NMDA) and cannabinoid CB2 receptors form functional complexes in cells of the central nervous system: insights into the therapeutic potential of neuronal and microglial NMDA receptors. Alzheimers Res Ther 13:184 (2021). PubMed: 34749800
- Tyrtyshnaia A et al. Synaptamide Improves Cognitive Functions and Neuronal Plasticity in Neuropathic Pain. Int J Mol Sci 22:N/A (2021). PubMed: 34884587