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Cell Biology Apoptosis Intracellular Associated Proteins

Anti-NLRP3抗体(ab4207)

  • Datasheet
  • SDS
Reviews (8)Q&A (1)References (88)

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Western blot - Anti-NLRP3 antibody (ab4207)
  • Flow Cytometry (Intracellular) - Anti-NLRP3 antibody (ab4207)
  • Flow Cytometry (Intracellular) - Anti-NLRP3 antibody (ab4207)

Key features and details

  • Goat polyclonal to NLRP3
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-NLRP3 antibody [EPR23073-96] (ab270449)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-NLRP3抗体
    参阅全部 NLRP3 一抗
  • 描述

    山羊多克隆抗体to NLRP3
  • 宿主

    Goat
  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details
    不适用于: ICC/IF
  • 种属反应性

    与反应: Human
    预测可用于: Rat, Cow
  • 免疫原

    Synthetic peptide corresponding to Human NLRP3 aa 1024-1036 (C terminal).
    Sequence:

    C-EKPELTVVFEPSW


    Database link: Q96P20-1
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • Flow Cyt (intra): U937 cells. WB: THP-1 cells
  • 常规说明

    No signal has been obtained in Western blot but low background has observed in Daudi, A431, Jurkat, U937 and MOLT-4 lysates at up to 1µg/ml. We would appreciate any feedback from people in the field.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Cancer
    • Growth factors
    • TNF
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human NLRP3 protein (ab165022)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4207于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use a concentration of 10 µg/ml.

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB (2)
1/1000. Predicted molecular weight: 118 kDa.
说明
Flow Cyt (Intra)
Use a concentration of 10 µg/ml.

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000. Predicted molecular weight: 118 kDa.
应用说明
Is unsuitable for ICC/IF.

靶标

  • 功能

    May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.
  • 组织特异性

    Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.
  • 疾病相关

    Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
    Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
    Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation.
  • 序列相似性

    Belongs to the NLRP family.
    Contains 1 DAPIN domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • 细胞定位

    Cytoplasm.
  • Target information above from: UniProt accession Q96P20 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 114548 Human
    • Entrez Gene: 287362 Rat
    • Omim: 606416 Human
    • SwissProt: Q96P20 Human
    • Unigene: 159483 Human
    • 别名

      • AGTAVPRL antibody
      • AII/AVP antibody
      • Angiotensin/vasopressin receptor AII/AVP like antibody
      • Angiotensin/vasopressin receptor AII/AVP-like antibody
      • C1orf7 antibody
      • Caterpiller protein 1.1 antibody
      • CIAS 1 antibody
      • CIAS1 antibody
      • CLR1.1 antibody
      • Cold autoinflammatory syndrome 1 antibody
      • Cold autoinflammatory syndrome 1 protein antibody
      • Cryopyrin antibody
      • Familial cold autoinflammatory syndrome antibody
      • FCAS antibody
      • FCU antibody
      • LRR and PYD domains-containing protein 3 antibody
      • Muckle-Wells syndrome antibody
      • MWS antibody
      • NACHT antibody
      • NACHT LRR and PYD containing protein 3 antibody
      • NALP 3 antibody
      • NALP3 antibody
      • NALP3_HUMAN antibody
      • NLR family pyrin domain containing 3 antibody
      • NLRP3 antibody
      • PYPAF 1 antibody
      • PYPAF1 antibody
      • PYRIN containing APAF1 like protein 1 antibody
      • PYRIN-containing APAF1-like protein 1 antibody
      see all

    图片

    • Western blot - Anti-NLRP3 antibody (ab4207)
      Western blot - Anti-NLRP3 antibody (ab4207)
      All lanes : Anti-NLRP3 antibody (ab4207) at 1/1000 dilution

      Lane 1 : Wild-type THP-1 cell lysate
      Lane 2 : NLRP3 knockout THP-1 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 118 kDa
      Observed band size: 118 kDa



      False colour image of Western blot: Anti-NLRP3 antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab4207 was shown to bind specifically to NLRP3. A band was observed at 118 kDa in wild-type THP-1 cell lysates with no signal observed at this size in NLRP3 knockout cell line ab280063 (knockout cell lysate ab280122). To generate this image, wild-type and NLRP3 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Donkey anti-Goat IgG H&L (IRDye® 800CW) preabsorbed (ab216775) and Donkey anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216778) at 1/20000 dilution.

    • Flow Cytometry (Intracellular) - Anti-NLRP3 antibody (ab4207)
      Flow Cytometry (Intracellular) - Anti-NLRP3 antibody (ab4207)

      Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab4207 was 1hr (10ug/ml) followed by Alexa Fluor® 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.

    • Flow Cytometry (Intracellular) - Anti-NLRP3 antibody (ab4207)
      Flow Cytometry (Intracellular) - Anti-NLRP3 antibody (ab4207)This image is courtesy of an Abreview submitted by Mahesh Shivananjappa.

      ab4207 staining NLRP3 in the Human White Blood Cells (Mixed Population) by Flow Cytometry. WBC were isolated spinning Blood on Ficoll Gradient after removal of RBC's and permeabilized with 0.1% Triton-X100 in 2% BSA for 15 minutes. The sample was incubated with the primary antibody (1/100 in PBS + 2% BSA in PBS) for 16 hours at 4°C. An Alexa Flour® 488 Donkey Anti Goat IgG (H+L) (1/250) was used as the secondary antibody.
      Gating Strategy: Monocytes

      See Abreview

    实验方案

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (88)

    发表研究结果有使用 ab4207?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4207 被引用在 88 文献中.

    • Qin Y  et al. Impaired autophagy in microglia aggravates dopaminergic neurodegeneration by regulating NLRP3 inflammasome activation in experimental models of Parkinson's disease. Brain Behav Immun 91:324-338 (2021). PubMed: 33039664
    • Chen Y  et al. NLRP3 regulates alveolar bone loss in ligature-induced periodontitis by promoting osteoclastic differentiation. Cell Prolif 54:e12973 (2021). PubMed: 33382502
    • Cheng Y  et al. iTRAQ-Based Quantitative Proteomics Indicated Nrf2/OPTN-Mediated Mitophagy Inhibits NLRP3 Inflammasome Activation after Intracerebral Hemorrhage. Oxid Med Cell Longev 2021:6630281 (2021). PubMed: 33628368
    • Liu X  et al. Bone marrow mesenchymal stem cell-derived exosomes attenuate cerebral ischemia-reperfusion injury-induced neuroinflammation and pyroptosis by modulating microglia M1/M2 phenotypes. Exp Neurol 341:113700 (2021). PubMed: 33741350
    • Zheng S  et al. microRNA-129 overexpression in endothelial cell-derived extracellular vesicle influences inflammatory response caused by myocardial ischemia/reperfusion injury. Cell Biol Int 45:1743-1756 (2021). PubMed: 33851774
    View all Publications for this product

    客户评价及客户问答

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-NLRP3 antibody

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    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human Primary Fibroblast)
    Permeabilization
    Yes - 0.1% Triton 1h
    Specification
    Human Primary Fibroblast
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    提交于 Nov 20 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-NLRP3 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (brain)
    Permeabilization
    Yes - triton
    Specification
    brain
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 30°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Jan 23 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-NLRP3 antibody

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    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Permeabilization
    Yes - 5% Triton-X in 0.05M Phosphate Buffered Saline
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Feb 28 2018

    Western blot abreview for Anti-NLRP3 antibody

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    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (macrophage)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Treatment
    0.1 ug/ml LPS 15 min
    Specification
    macrophage
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Dec 12 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CIAS1 / NALP3 antibody

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    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human MDM)
    Specification
    human MDM
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - TRITON-X (0.1 %)
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Feb 06 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CIAS1 / NALP3 antibody

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    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (RAW 264.7)
    Specification
    RAW 264.7
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton-X100 in 2% BSA for 15min
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Mahesh Shivananjappa

    Verified customer

    提交于 May 24 2012

    Western blot abreview for Anti-CIAS1 / NALP3 antibody

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    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Corpus Cavernosum)
    Loading amount
    20 µg
    Specification
    Corpus Cavernosum
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Jason Davis

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    提交于 Jan 13 2012

    Flow Cytometry abreview for Anti-CIAS1 / NALP3 antibody

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    Application
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    Sample
    Human Cell (White Blood Cells (Mixed Population))
    Specification
    White Blood Cells (Mixed Population)
    Preparation
    Cell harvesting/tissue preparation method: WBC were isolated spinning Blood on Ficoll Gradient after removal of RBC's
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton-X100 in 2% BSA for 15min
    Gating Strategy
    Monocytes
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Mahesh Shivananjappa

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    提交于 Feb 15 2011

    Question

    I would like to test ab4207 for use on canine samples.

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    DISCOUNT CODE: *** Expiration date: February 29th, 2013 Value: *** I am very pleased to hear you would like to accept our offer and test ab4207 in canine. This code will give you *** off your next order before the expiration date. To redeem this offer, please submit an Abreview for canine and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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