AB325273

Anti-NLRC5 antibody [EPR29722-55]

RabMAb
Recombinant
20ul selling size
了解详情

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Rabbit Recombinant Monoclonal NLRC5 antibody. Suitable for WB and reacts with Human samples.

NOD27, NOD4, NLRC5, Protein NLRC5, Caterpiller protein 16.1, Nucleotide-binding oligomerization domain protein 27, Nucleotide-binding oligomerization domain protein 4, CLR16.1

2 Images

Lab

Western blot - Anti-NLRC5 antibody [EPR29722-55] (AB325273)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of NLRC5 is upregulated in response to IFN gamma treatment (PMID : 36103539; PMID : 20610642; PMID : 20061403).

This antibody can recognize the different isoforms of NLRC5.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-NLRC5 antibody [EPR29722-55] (ab325273) at 1/1000 dilution

Lane 1:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa treated with 1000U/ml IFN gamma for 6 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 205 kDa,170 kDa,124 kDa

false

Exposure time: 180s

Lab

Western blot - Anti-NLRC5 antibody [EPR29722-55] (AB325273)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of NLRC5 is upregulated in response to IFN gamma treatment (PMID : 36103539; PMID : 20610642; PMID : 20061403).

This antibody can recognize the different isoforms of NLRC5.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-NLRC5 antibody [EPR29722-55] (ab325273) at 1/1000 dilution

Lane 1:

Untreated Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

Lane 2:

Jurkat treated with 10ng/ml IFN gamma for 24 hours whole cell lysate at 20 µg

Lane 3:

Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 4:

THP-1 treated with 10ng/ml IFN gamma for 24 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 205 kDa,170 kDa,124 kDa

false

Exposure time: 114s

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR29722-55

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Probable regulator of the NF-kappa-B and type I interferon signaling pathways. May also regulate the type II interferon signaling pathway. Plays a role in homeostatic control of innate immunity and in antiviral defense mechanisms.

See full target information NLRC5

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NLRC5 antibody [EPR29722-55]