Anti-NLN抗体[OTI1D6] (ab119802)
![Western blot - Anti-NLN antibody [OTI1D6] (ab119802)](https://www.abcam.cn/ps/products/119/ab119802/Images/NLN-Primary-antibodies-ab119802-1.jpg "Western blot - Anti-NLN antibody [OTI1D6] (ab119802)")
Key features and details
- Mouse monoclonal [OTI1D6] to NLN
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
概述
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产品名称
Anti-NLN抗体[OTI1D6] -
描述
小鼠单克隆抗体[OTI1D6] to NLN -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
种属反应性
与反应: Mouse, Rat, Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human NLN aa 1-750. Produced in HEK293T cells (NP_065777).
Database link: Q9BYT8 -
阳性对照
- WB: HEK-293T cells transfected with NLN, HepG2, HeLa, SVT2, A549, COS7, PC12, and MCF7 cell extracts. IHC-P: Carcinoma of Human pancreas, Adenocarcinoma of Human endometrium, Carcinoma of Human bladder and Human tonsil tissues. ICC/IF: COS7 cells transiently transfected with NLN. Flow Cyt (Intra): Jurkat cells.
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常规说明
The clone number has been updated from 1D6 to OTI1D6, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography. -
克隆
单克隆 -
克隆编号
OTI1D6 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119802于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 81 kDa.
|
|
| IHC-P |
1/150.
|
|
| ICC/IF |
1/100.
|
|
| Flow Cyt (Intra) |
1/100.
|
| 说明 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 81 kDa. |
|
IHC-P
1/150. |
|
ICC/IF
1/100. |
|
Flow Cyt (Intra)
1/100. |
靶标
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功能
Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A. -
序列相似性
Belongs to the peptidase M3 family. -
细胞定位
Mitochondrion intermembrane space. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 57486 Human
- Entrez Gene: 75805 Mouse
- Entrez Gene: 117041 Rat
- Omim: 611530 Human
- SwissProt: Q9BYT8 Human
- SwissProt: Q91YP2 Mouse
- SwissProt: P42676 Rat
- Unigene: 247460 Human
see all -
别名
- AGTBP antibody
- Angiotensin binding protein antibody
- Angiotensin-binding protein antibody
see all
图片
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Western blot - Anti-NLN antibody [OTI1D6] (ab119802)All lanes : Anti-NLN antibody [OTI1D6] (ab119802) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY NLN cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 81 kDa -
![Western blot - Anti-NLN antibody [OTI1D6] (ab119802)](https://www.abcam.cn/ps/products/119/ab119802/Images/NLN-Primary-antibodies-ab119802-2.jpg)
Western blot - Anti-NLN antibody [OTI1D6] (ab119802)All lanes : Anti-NLN antibody [OTI1D6] (ab119802) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : SVT2 cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 7 : MDCK cell extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line)
cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 81 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (ab119802)](https://www.abcam.cn/ps/products/119/ab119802/Images/NLN-Primary-antibodies-ab119802-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (ab119802)Paraffin-embedded human pancreas carcinoma tissue stained for NLN using ab119802 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (ab119802)](https://www.abcam.cn/ps/products/119/ab119802/Images/NLN-Primary-antibodies-ab119802-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (ab119802)Paraffin-embedded human endometrium adenocarcinoma tissue stained for NLN using ab119802 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (ab119802)](https://www.abcam.cn/ps/products/119/ab119802/Images/NLN-Primary-antibodies-ab119802-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (ab119802)Paraffin-embedded human bladder carcinoma tissue stained for NLN using ab119802 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (ab119802)](https://www.abcam.cn/ps/products/119/ab119802/Images/NLN-Primary-antibodies-ab119802-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (ab119802)Paraffin-embedded human tonsil tissue stained for NLN using ab119802 at 1/150 dilution in immunohistochemical analysis.
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![Immunocytochemistry/ Immunofluorescence - Anti-NLN antibody [OTI1D6] (ab119802)](https://www.abcam.cn/ps/products/119/ab119802/Images/NLN-Primary-antibodies-ab119802-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NLN antibody [OTI1D6] (ab119802)COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY NLN, stained for NLN (green) using ab119802 at 1/100 dilution in ICC/IF.
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![Flow Cytometry (Intracellular) - Anti-NLN antibody [OTI1D6] (ab119802)](https://www.abcam.cn/ps/products/119/ab119802/Images/ab119802-314133-143.jpg)
Flow Cytometry (Intracellular) - Anti-NLN antibody [OTI1D6] (ab119802)Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells using ab119802 at 1/100 dilution stained for NLN (Red) compared to a nonspecific negetive control antibody (Blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab119802 被引用在 1 文献中.
- Tsai KW et al. Arm Selection Preference of MicroRNA-193a Varies in Breast Cancer. Sci Rep 6:28176 (2016). WB . PubMed: 27307030