Anti-Nkx2.5抗体(ab106923)
Key features and details
- Goat polyclonal to Nkx2.5
- Suitable for: WB, ICC, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Nkx2.5抗体
参阅全部 Nkx2.5 一抗 -
描述
山羊多克隆抗体to Nkx2.5 -
宿主
Goat -
特异性
ab106923 is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
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经测试应用
适用于: WB, ICC, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human Nkx2.5 aa 50-150 (internal sequence). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- WB: Mouse heart lysate; ICC: U-2 OS cells. IHC: Human heart.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab106923 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106923于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 35 kDa).
A 1 hour primary incubation is recommended for this product. Abcam recommends using 3% milk as the blocking agent. |
| ICC |
Use a concentration of 10 µg/ml.
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| IHC-P |
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Only suitable for human, not mouse samples. |
| 说明 |
|---|
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 35 kDa). A 1 hour primary incubation is recommended for this product. Abcam recommends using 3% milk as the blocking agent. |
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ICC
Use a concentration of 10 µg/ml. |
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IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Only suitable for human, not mouse samples. |
靶标
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功能
Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development. -
组织特异性
Expressed only in the heart. -
疾病相关
Atrial septal defect 7, with or without atrioventricular conduction defects
Tetralogy of Fallot
Conotruncal heart malformations
Hypothyroidism, congenital, non-goitrous, 5
Ventricular septal defect 3
Hypoplastic left heart syndrome 2
Asplenia, isolated congenital -
序列相似性
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1482 Human
- Entrez Gene: 18091 Mouse
- Omim: 600584 Human
- SwissProt: P52952 Human
- SwissProt: P42582 Mouse
- Unigene: 54473 Human
- Unigene: 41974 Mouse
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别名
- NKX2.5, mouse, homolog of antibody
- Cardiac-specific homeobox 1 antibody
- Cardiac-specific homeobox antibody
see all
图片
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Western blot - Anti-Nkx2.5 antibody (ab106923)All lanes : Anti-Nkx2.5 antibody (ab106923) at 1 µg/ml
Lane 1 : Human heart lysate in RIPA buffer
Lane 2 : Mouse heart lysate in RIPA buffer
Lane 3 : Rat heart lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 35 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
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Immunocytochemistry - Anti-Nkx2.5 antibody (ab106923)Immunocytochemistry analysis of paraformaldehyde fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells, permeabilized with 0.15% Triton. Primary incubation for 1 hour at 10 µg/mL followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.5 antibody (ab106923)Immunohistochemical analysis of human heart tissue stained for Nkx2.5 using ab106923 at 4µg/ml. Positive cytoplasmic staining shown.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab106923 被引用在 7 文献中.
- Swift SK et al. Cardiomyocyte ploidy is dynamic during postnatal development and varies across genetic backgrounds. Development 150:N/A (2023). PubMed: 36912240
- Zhu D et al. Minimally invasive delivery of therapeutic agents by hydrogel injection into the pericardial cavity for cardiac repair. Nat Commun 12:1412 (2021). PubMed: 33658506
- Yu B et al. Circular RNA hsa_circ_105039 promotes cardiomyocyte differentiation by sponging miR‑17 to regulate cyclinD2 expression. Mol Med Rep 24:N/A (2021). PubMed: 34664684
- Chatterjee S & Chakravarti A A gene regulatory network explains RET-EDNRB epistasis in Hirschsprung disease. Hum Mol Genet 28:3137-3147 (2019). PubMed: 31313802
- Chen J et al. Nkx2.5 insufficiency leads to atrial electrical remodeling through Wnt signaling in HL-1 cells. Exp Ther Med 18:4631-4636 (2019). PubMed: 31798700
- Liu H et al. Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis. Stem Cells Int 2016:2524092 (2016). PubMed: 26798354
- Mohammadi A et al. Generation of Rat Embryonic Germ Cells via Inhibition of TGFß and MEK Pathways. Cell J 17:288-95 (2015). Rat . PubMed: 26199907