Anti-Nkx2.2抗体(ab272915)
Key features and details
- Rabbit polyclonal to Nkx2.2
- Suitable for: WB, IHC-Fr, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
概述
-
产品名称
Anti-Nkx2.2抗体
参阅全部 Nkx2.2 一抗 -
描述
兔多克隆抗体to Nkx2.2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Fr, ICC/IFmore details -
种属反应性
与反应: Rat, Human -
免疫原
Recombinant fragment within Mouse Nkx2.2 (C terminal). The exact sequence is proprietary.
Database link: P42586 -
阳性对照
- IHC-Fr: Rat E13.5 brain tissue. ICC/IF: DIV9 rat E18 primary hippocampal neuron cells. WB: HEK-293T cells transfected with DDDDK-tagged Nkx2.2.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272915于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000.
|
|
| IHC-Fr |
1/100 - 1/1000.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. |
|
IHC-Fr
1/100 - 1/1000. |
|
ICC/IF
1/100 - 1/1000. |
靶标
-
功能
May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. -
序列相似性
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
结构域
The homeodomain is essential for interaction with OLIG2. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 4821 Human
- Entrez Gene: 366214 Rat
- Omim: 604612 Human
- SwissProt: O95096 Human
- Unigene: 516922 Human
-
别名
- Homeobox protein NK 2 homolog B antibody
- Homeobox protein NK-2 homolog B antibody
- Homeobox protein Nkx 2.2 antibody
see all
图片
-
Immunohistochemistry (Frozen sections) - Anti-Nkx2.2 antibody (ab272915)
Paraffin-embedded rat E13.5 brain tissue stained for Nkx2.2 using ab272915 at 1/250 dilution in immunohistochemical analysis.
Red: Beta tubulin.
Blue: DAPI.
-
Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.2 antibody (ab272915)4% PFA-fixed DIV9 rat E18 primary hippocampal neuron cells stained for Nkx2.2 using ab272915 at 1/500 dilution in ICC/IF.
Red: Beta tubulin.
Blue: DAPI.
-
Western blot - Anti-Nkx2.2 antibody (ab272915)All lanes : Anti-Nkx2.2 antibody (ab272915) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T cell lysate
Lane 2 : DDDDK-tagged Nkx2.2 transfected HEK-293T cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody12% SDS-PAGE.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab272915 尚未被引用在任何文献中。