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AB324533

Anti-NKG2A antibody [EPR27255-560] - BSA and Azide free

Anti-NKG2A antibody [EPR27255-560] - BSA and Azide free

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Rabbit Recombinant Monoclonal NKG2A antibody. Carrier free. Suitable for Dot, Flow Cyt and reacts with Transfected cell lysate - Human, Human samples.

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CD159a, NKG2A, KLRC1, NKG2-A/NKG2-B type II integral membrane protein, CD159 antigen-like family member A, NK cell receptor A, NKG2-A/B-activating NK receptor

4 Images
Flow Cytometry - Anti-NKG2A antibody [EPR27255-560] - BSA and Azide free (AB324533)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-NKG2A antibody [EPR27255-560] - BSA and Azide free (AB324533)

This data was developed using ab324524, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling NKG2A with ab324524 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells are co-stained with CD56 conjugated to Brilliant Violet 421.

Flow Cytometry - Anti-NKG2A antibody [EPR27255-560] - BSA and Azide free (AB324533)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-NKG2A antibody [EPR27255-560] - BSA and Azide free (AB324533)

This data was developed using ab324524, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling NKG2A with ab324524 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells are co-stained with CD14 conjugated to Alexa Fluor® 488.

Flow Cytometry - Anti-NKG2A antibody [EPR27255-560] - BSA and Azide free (AB324533)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-NKG2A antibody [EPR27255-560] - BSA and Azide free (AB324533)

This data was developed using ab324524, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell, Left) / No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag, Right) cells labelling NKG2A with ab324524 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Negative control : HeLa.

Dot Blot - Anti-NKG2A antibody [EPR27255-560] - BSA and Azide free (AB324533)
  • Dot

Supplier Data

Dot Blot - Anti-NKG2A antibody [EPR27255-560] - BSA and Azide free (AB324533)

This data was developed using ab324524, the same antibody clone in a different buffer formulation.

Dot blot analysis of NKG2A using ab324524 at 1 : 1000 (0.47 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : 293T (human embryonic kidney epithelial cell) cells transfected with a human NKG2A expression vector containing a myc-His-tag®, whole cell lysate
Lane2 : 293T cells transfected with a human NKG2C expression vector containing a myc-His-tag®, whole cell lysate
Lane3 : 293T cells transfected with a human NKG2E expression vector containing a myc-His-tag®, whole cell lysate

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human NKG2C or NKG2E by dot blot.

In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Dot Blot - Anti-NKG2A antibody [EPR27255-560] (<a href='/products/primary-antibodies/nkg2a-antibody-epr27255-560-ab324524'>ab324524</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with a human NKG2A expression vector containing a myc-His-tag®, whole cell lysate

Lane 2:

293T cells transfected with a human NKG2C expression vector containing a myc-His-tag®, whole cell lysate

Lane 3:

293T cells transfected with a human NKG2E expression vector containing a myc-His-tag®, whole cell lysate

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR27255-560

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

Dot, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody does not cross-react with overexpressed human NKG2C or NKG2E by dot blot.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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产品详情

ab324533 is the carrier-free version of ab324524.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Immune inhibitory receptor involved in self-nonself discrimination. In complex with KLRD1 on cytotoxic and regulatory lymphocyte subsets, recognizes non-classical major histocompatibility (MHC) class Ib molecule HLA-E loaded with self-peptides derived from the signal sequence of classical MHC class Ia molecules. Enables cytotoxic cells to monitor the expression of MHC class I molecules in healthy cells and to tolerate self (PubMed : 18083576, PubMed : 37264229, PubMed : 9430220, PubMed : 9486650). Upon HLA-E-peptide binding, transmits intracellular signals through two immunoreceptor tyrosine-based inhibition motifs (ITIMs) by recruiting INPP5D/SHP-1 and INPPL1/SHP-2 tyrosine phosphatases to ITIMs, and ultimately opposing signals transmitted by activating receptors through dephosphorylation of proximal signaling molecules (PubMed : 12165520, PubMed : 9485206). Key inhibitory receptor on natural killer (NK) cells that regulates their activation and effector functions (PubMed : 30860984, PubMed : 9430220, PubMed : 9485206, PubMed : 9486650). Dominantly counteracts T cell receptor signaling on a subset of memory/effector CD8-positive T cells as part of an antigen-driven response to avoid autoimmunity (PubMed : 12387742). On intraepithelial CD8-positive gamma-delta regulatory T cells triggers TGFB1 secretion, which in turn limits the cytotoxic programming of intraepithelial CD8-positive alpha-beta T cells, distinguishing harmless from pathogenic antigens (PubMed : 18064301). In HLA-E-rich tumor microenvironment, acts as an immune inhibitory checkpoint and may contribute to progressive loss of effector functions of NK cells and tumor-specific T cells, a state known as cell exhaustion (PubMed : 30503213, PubMed : 30860984).. (Microbial infection) Viruses like human cytomegalovirus have evolved an escape mechanism whereby virus-induced down-regulation of host MHC class I molecules is coupled to the binding of viral peptides to HLA-E, restoring HLA-E expression and inducing HLA-E-dependent NK cell immune tolerance to infected cells. Recognizes HLA-E in complex with human cytomegalovirus UL40-derived peptide (VMAPRTLIL) and inhibits NK cell cytotoxicity.. (Microbial infection) May recognize HLA-E in complex with HIV-1 gag/Capsid protein p24-derived peptide (AISPRTLNA) on infected cells and may inhibit NK cell cytotoxicity, a mechanism that allows HIV-1 to escape immune recognition.. (Microbial infection) Upon SARS-CoV-2 infection, may contribute to functional exhaustion of cytotoxic NK cells and CD8-positive T cells (PubMed : 32203188, PubMed : 32859121). On NK cells, may recognize HLA-E in complex with SARS-CoV-2 S/Spike protein S1-derived peptide (LQPRTFLL) expressed on the surface of lung epithelial cells, inducing NK cell exhaustion and dampening antiviral immune surveillance (PubMed : 32859121).
See full target information KLRC1
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