重组Anti-NKG2A + NKG2E + NKG2C抗体[EPR22137] (ab224699)
![Western blot - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)](https://www.abcam.cn/ps/products/224/ab224699/Images/ab224699-346695-anti-nkg2A--nkg2E--nkg2C-antibody-epr22137-western-blot-human-hek-293t.png "Western blot - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22137] to NKG2A + NKG2E + NKG2C
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-NKG2A + NKG2E + NKG2C抗体[EPR22137] -
描述
兔单克隆抗体[EPR22137] to NKG2A + NKG2E + NKG2C -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human endometrium WB: HEK-293T cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR22137 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224699于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 34-38 kDa (predicted molecular weight: 26 kDa).
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| IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 34-38 kDa (predicted molecular weight: 26 kDa). |
|
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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细胞定位
NKG2A: Membrane. NKG2E: Membrane. NKG2C: Membrane. -
数据库链接
- Entrez Gene: 3821 Human
- Entrez Gene: 3822 Human
- Entrez Gene: 3823 Human
- Omim: 161555 Human
- Omim: 602891 Human
- Omim: 602892 Human
- SwissProt: P26715 Human
- SwissProt: P26717 Human
see all
图片
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Western blot - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)All lanes : Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699) at 1/1000 dilution
Lanes 1 & 3 & 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), transfected with an empty vector (vector control), whole cell lysate with 5% NFDM/TBST
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), transfected with human His-tagged NKG2A (aa1-233) expression vector, whole cell lysate with 5% NFDM/TBST
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), transfected with human His-tagged NKG2C (aa1-231) expression vector, whole cell lysate with 5% NFDM/TBST
Lane 6 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), transfected with human His-tagged NKG2E (aa1-240) expression vector, whole cell lysate. with 5% NFDM/TBST
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsCompared with NKG2A, ab224699 has lower affinity with NKG2C and 2E.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)](https://www.abcam.cn/ps/products/224/ab224699/Images/ab224699-323590-anti-nkg2a-antibody-epr22137-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling NKG2A + NKG2E + NKG2C with ab224699 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on a subset of immune cells in human endometrium is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)](https://www.abcam.cn/ps/products/224/ab224699/Images/ab224699-5-benefits-of-recombinant-antibodies.png)
Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab224699 尚未被引用在任何文献中。