Anti-NKF3 kinase family member/PEAK1抗体(ab121865)
Key features and details
- Rabbit polyclonal to NKF3 kinase family member/PEAK1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NKF3 kinase family member/PEAK1抗体 -
描述
兔多克隆抗体to NKF3 kinase family member/PEAK1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human NKF3 kinase family member/PEAK1 aa 81-166.
Sequence:DGQSICGELSIQEHCENKPVIIGWNRNRAALSQKPLNNNNEDDEGISHVP KPYGNNDSAKKMSDNNNGLTEVLKEIAGLDTAPQIR
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阳性对照
- Human stomach tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121865于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
| 说明 |
|---|
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IHC-P
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
靶标
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功能
Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN. -
序列相似性
Belongs to the protein kinase superfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation. Autophosphorylated in vitro. -
细胞定位
Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Colocalizes with F-actin in serum-rich medium. Actin colocalization is reduced during serum starvation. - Information by UniProt
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数据库链接
- Entrez Gene: 79834 Human
- Omim: 614248 Human
- SwissProt: Q9H792 Human
- Unigene: 9587 Human
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别名
- FLJ21140 antibody
- FLJ34483 antibody
- KIAA2002 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-NKF3 kinase family member/PEAK1 antibody (ab121865)Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121865 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKF3 kinase family member/PEAK1 antibody (ab121865)ab121865, at a 1/10 dilution, staining NKF3 kinase family member/PEAK1 in glandular cells of paraffin-embedded Human stomach tissue by immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab121865 尚未被引用在任何文献中。