重组Anti-NK-p44抗体[EPR5060(2)] (ab133668)
![Western blot - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)](https://www.abcam.cn/ps/products/133/ab133668/Images/ab133668-147635-anti-nk-p44-antibody-epr50602-western-blot.jpg "Western blot - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5060(2)] to NK-p44
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-NK-p44抗体[EPR5060(2)]
参阅全部 NK-p44 一抗 -
描述
兔单克隆抗体[EPR5060(2)] to NK-p44 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human NK-p44 aa 1-100 (N terminal). The exact sequence is proprietary.
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阳性对照
- Human lymph node lysate; K562, Jurkat, HuT-78 and Molt-4 cell lysates; K562 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.00 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5060(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133668于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 31 kDa.
|
|
| ICC/IF |
1/100 - 1/250.
|
| 说明 |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 31 kDa. |
|
ICC/IF
1/100 - 1/250. |
应用说明
Is unsuitable for IHC-P or IP.
靶标
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功能
Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. -
组织特异性
Selectively expressed by activated NK cells and by in vitro cultured (i.e. activated) TCRg/d lymphoid cells. -
序列相似性
Belongs to the natural cytotoxicity receptor (NCR) family.
Contains 1 Ig-like (immunoglobulin-like) domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9436 Human
- Omim: 604531 Human
- SwissProt: O95944 Human
- Unigene: 194721 Human
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别名
- Activating NK receptor NKp44 antibody
- CD336 antibody
- CD336 antigen antibody
see all
图片
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Western blot - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)All lanes : Anti-NK-p44 antibody [EPR5060(2)] (ab133668) at 1/10000 dilution
Lane 1 : Human lymph node lysate
Lane 2 : K562 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HuT-78 cell lysate
Lane 5 : Molt-4 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution
Predicted band size: 31 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)](https://www.abcam.cn/ps/products/133/ab133668/Images/ab133668-147634-anti-nk-p44-antibody-epr50602-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)Immunofluorescent analysis of K562 cells labelling NK-p44 with ab133668 at 1/100 dilution.
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![OI-RD Scanning - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)](https://www.abcam.cn/ps/products/133/ab133668/Images/ab133668-147633-anti-nk-p44-antibody-epr50602.jpg)
OI-RD Scanning - Anti-NK-p44 antibody [EPR5060(2)] (ab133668) -
![Anti-NK-p44 antibody [EPR5060(2)] (ab133668)](https://www.abcam.cn/ps/products/133/ab133668/Images/ab133668-4-benefits-of-recombinant-antibodies.png)
Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab133668 被引用在 3 文献中.
- Adithan A et al. Biological effects of cyclosporin A on CD3-CD161+ and CD3+CD161+ lymphocytes. Mol Cell Biochem N/A:N/A (2019). PubMed: 31020492
- Qiu Y et al. Lactobacillus plantarum Enhanced IL-22 Production in Natural Killer (NK) Cells That Protect the Integrity of Intestinal Epithelial Cell Barrier Damaged by Enterotoxigenic Escherichia coli. Int J Mol Sci 18:N/A (2017). PubMed: 29137183
- Vourc'h M et al. Exoenzyme T Plays a Pivotal Role in the IFN-? Production afterPseudomonasChallenge in IL-12 Primed Natural Killer Cells. Front Immunol 8:1283 (2017). PubMed: 29067027