Anti-NK-1R抗体(ab61705)
Key features and details
- Goat polyclonal to NK-1R
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-NK-1R抗体
参阅全部 NK-1R 一抗 -
描述
山羊多克隆抗体to NK-1R -
宿主
Goat -
特异性
This antibody is expected to recognise both reported isoforms (NP_001049.1 and NP_056542.1). -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Dog
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免疫原
Synthetic peptide corresponding to Human NK-1R aa 227-239 (internal sequence).
Sequence:C-EIPGDSSDRYHEQ
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阳性对照
- WB: HeLa cell lysate and Neuro2a cell lysate. ICC/IF: Neuro2a cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab61705于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| ICC/IF |
| 说明 |
|---|
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight isoform 2: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 403815 Dog
- Entrez Gene: 6869 Human
- Entrez Gene: 21336 Mouse
- Omim: 162323 Human
- SwissProt: Q5DUB3 Dog
- SwissProt: P25103 Human
- SwissProt: P30548 Mouse
- Unigene: 633301 Human
see all -
别名
- Neurokinin 1 antibody
- Neurokinin 1 Receptor antibody
- NK 1 receptor antibody
see all
图片
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Western blot - Anti-NK-1R antibody (ab61705)Lane 1 : Anti-NK-1R antibody (ab61705) at 1 µg/ml
Lane 2 : Anti-NK-1R antibody (ab61705) at 0.5 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 46 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Lane 1-2: ab61705 (1µg/ml) staining of HeLa (A) and (0.5ug/ml) Neuro2a (B) cell lysate.
Primary incubation 1 hour at room temperature. Detected by chemiluminescence. RIPA buffer used.
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Immunocytochemistry/ Immunofluorescence - Anti-NK-1R antibody (ab61705)Immunofluorescence analysis of paraformaldehyde fixed, 0.15% Triton permeabilized Neuro2a (Mouse neuroblastoma cell line) cells labelling NK-1R with primary Anti-NK-1R antibody (ab61705) at 10ug/ml for 1 hour, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. Image showing nuclear and plasma membrane staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG at 10ug/ml followed by Alexa Fluor 488 secondary antibody at 2ug/ml.
数据表及文件
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Datasheet download
文献 (4)
ab61705 被引用在 4 文献中.
- Lasagni Vitar R et al. Substance P/neurokinin-1 receptor pathway blockade ameliorates limbal stem cell deficiency by modulating mTOR pathway and preventing cell senescence. Stem Cell Reports 17:849-863 (2022). PubMed: 35334220
- Lasagni Vitar RM et al. Modulating Ocular Surface Pain Through Neurokinin-1 Receptor Blockade. Invest Ophthalmol Vis Sci 62:26 (2021). PubMed: 33729475
- Lasagni Vitar RM et al. A Hypothalamic-Controlled Neural Reflex Promotes Corneal Inflammation. Invest Ophthalmol Vis Sci 62:21 (2021). PubMed: 34698773
- Liu X et al. Antagonism of NK-1R using aprepitant suppresses inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes. Artif Cells Nanomed Biotechnol 47:1628-1634 (2019). PubMed: 31010320
