Anti-Nitro tyrosine抗体[HM.11] (ab7048)
Key features and details
- Mouse monoclonal [HM.11] to Nitro tyrosine
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Species independent
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
Anti-Nitro tyrosine抗体[HM.11]
参阅全部 Nitro tyrosine 一抗 -
描述
小鼠单克隆抗体[HM.11] to Nitro tyrosine -
宿主
Mouse -
特异性
This antibody reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. The antibody does not recognize other antigens with a nitrotyrosine-like structure such as tyrosine, 3-chlorotyrosine, phosphotyrosine and phenylalanine. -
经测试应用
适用于: IHC-Fr, IHC-Pmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to Nitro tyrosine (nitrated ) conjugated to Keyhole Limpet Haemocyanin (KLH).
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阳性对照
- WB: mouse kidney lysate, mouse optic nerve, retina , spinal cord and brain lysates, rat aorta lysate(see Abreviews) IHC-P: human lung tissue (see Abreview)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituents: PBS, 0.1% BSA
WARNING!!: Do not add sodium azide or nitric containing perservative as it will damage the antibody. -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
HM.11 -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
Acetone fixation 10 min -20 degree C; block endogenous peroxidase by 0.3 % H2O2 in PBS (or methanol for intracellular staining); block with 10% NGS or 5 % BSA for 30 min. |
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IHC-P | (3) |
1/200 - 1/400. 10% formalin fixation; 3% H2O2 to block endogenous peroxidases; Citrate buffer pH 6.0 for 1 min at 100°C as antigen retrieval treatment
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说明 |
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IHC-Fr
Use at an assay dependent concentration. Acetone fixation 10 min -20 degree C; block endogenous peroxidase by 0.3 % H2O2 in PBS (or methanol for intracellular staining); block with 10% NGS or 5 % BSA for 30 min. |
IHC-P
1/200 - 1/400. 10% formalin fixation; 3% H2O2 to block endogenous peroxidases; Citrate buffer pH 6.0 for 1 min at 100°C as antigen retrieval treatment |
靶标
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相关性
Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques. -
别名
- NO tyrosine antibody
- nTyr antibody
图片
数据表及文件
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SDS download
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Datasheet download
文献 (70)
ab7048 被引用在 70 文献中.
- Tryfonos A et al. Association between atherogenic risk-modulating proteins and endothelium-dependent flow-mediated dilation in coronary artery disease patients. Eur J Appl Physiol 123:367-380 (2023). PubMed: 36305972
- D'Haese S et al. Acute Exposure to Glycated Proteins Impaired in the Endothelium-Dependent Aortic Relaxation: A Matter of Oxidative Stress. Int J Mol Sci 23:N/A (2022). PubMed: 36499244
- Jang J et al. Interleukin-4 Aggravates LPS-Induced Striatal Neurodegeneration In Vivo via Oxidative Stress and Polarization of Microglia/Macrophages. Int J Mol Sci 23:N/A (2022). PubMed: 35008995
- Pereira PDS et al. Haptoglobin treatment contributes to regulating nitric oxide signal and reduces oxidative stress in the penis: A preventive treatment for priapism in sickle cell disease. Front Physiol 13:961534 (2022). PubMed: 36176769
- Liou GG et al. N-Acetyl Cysteine Overdose Inducing Hepatic Steatosis and Systemic Inflammation in Both Propacetamol-Induced Hepatotoxic and Normal Mice. Antioxidants (Basel) 10:N/A (2021). PubMed: 33809388