Anti-NIPSNAP1抗体(ab67302)
Key features and details
- Rabbit polyclonal to NIPSNAP1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-NIPSNAP1抗体
参阅全部 NIPSNAP1 一抗 -
描述
兔多克隆抗体to NIPSNAP1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human Brain Tissue Lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab67302于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| IHC-P |
Use a concentration of 2.5 µg/ml.
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| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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| 说明 |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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IHC-P
Use a concentration of 2.5 µg/ml. |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
靶标
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相关性
NIPSNAP is a member of an evolutionarily well conserved gene family and has a strong sequence similarity to the central portion of a protein encoded by C. elegans chromosome III between a 4-nitrophenylphosphatase (NIP) domain and non-neuronal SNAP25-like protein. Recent studies have indicated that NIPSNAP is involved in the regulation of the Ca2+-selective transient receptor potential vanilloid channel 6 (TRPV6). NIPSNAP1 associates with TRPV6 at the plasma membrane and inhibits TRPV6 currents. Other studies show that NIPSNAP’s expression is reduced in the phenylketonuria (PKU) mouse brain, suggesting that NIPSNAP may play a role in memory. -
细胞定位
Mitochondrial -
数据库链接
- Entrez Gene: 8508 Human
- Entrez Gene: 18082 Mouse
- Entrez Gene: 360971 Rat
- Omim: 603249 Human
- SwissProt: Q9BPW8 Human
- SwissProt: O55125 Mouse
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别名
- NIPSNAP 1 antibody
- Nipsnap homolog 1 (C. elegans) antibody
- Protein NipSnap homolog 1 antibody
图片
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Western blot - Anti-NIPSNAP1 antibody (ab67302)Lane 1 : Anti-NIPSNAP1 antibody (ab67302) at 0.5 µg/ml
Lane 2 : Anti-NIPSNAP1 antibody (ab67302) at 1 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 33 kDa
Observed band size: 33 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIPSNAP1 antibody (ab67302)ab67302 at 2.5 µg/ml staining NIPSNAP1 in human prostate tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
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Immunocytochemistry/ Immunofluorescence - Anti-NIPSNAP1 antibody (ab67302)Immunofluorescence of NIPSNAP in Human Brain cells using ab67302 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab67302 被引用在 3 文献中.
- Munson MJ et al. GAK and PRKCD are positive regulators of PRKN-independent mitophagy. Nat Commun 12:6101 (2021). PubMed: 34671015
- Princely Abudu Y et al. NIPSNAP1 and NIPSNAP2 Act as "Eat Me" Signals for Mitophagy. Dev Cell 49:509-525.e12 (2019). PubMed: 30982665
- Uthaiah RC & Hudspeth AJ Molecular anatomy of the hair cell's ribbon synapse. J Neurosci 30:12387-99 (2010). PubMed: 20844134