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Signal Transduction Protein Phosphorylation Ser / Thr Phosphatases

Anti-NIPP1抗体(ab5300)

  • Datasheet
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Western blot - Anti-NIPP1 antibody (ab5300)

    Key features and details

    • Goat polyclonal to NIPP1
    • Suitable for: IHC-P, WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-NIPP1抗体
      参阅全部 NIPP1 一抗
    • 描述

      山羊多克隆抗体to NIPP1
    • 宿主

      Goat
    • 经测试应用

      适用于: IHC-P, WBmore details
    • 种属反应性

      与反应: Mouse, Human
      预测可用于: Rat, Cow, Dog
    • 免疫原

      Synthetic peptide corresponding to Human NIPP1 aa 114-127 (C terminal).
      Sequence:

      C-EAWPGKKPTPSLLI


      (Peptide available as ab23096)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 阳性对照

      • WB: HeLa, HepG2, Jurkat and NIH3T3 nuclear lysates. IHC-P: Human thymus tissue.
    • 常规说明


      This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Primary antibody说明

      This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Phosphatases
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • RNA Processing
      • Splicing
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Other factors
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Kinases/Phosphatases
      • Other
      • Epigenetics and Nuclear Signaling
      • Chromatin Binding Proteins
      • DNA / RNA binding

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant Human NIPP1 protein (ab131705)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab5300于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    Use a concentration of 2.5 - 3.8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    WB
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

    1 hour primary incubation is recommended for this product.

    说明
    IHC-P
    Use a concentration of 2.5 - 3.8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    WB
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

    1 hour primary incubation is recommended for this product.

    靶标

    • 功能

      Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.
      Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.
    • 组织特异性

      Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells.
    • 序列相似性

      Contains 1 FHA domain.
    • 结构域

      Has a basic N- and C-terminal and an acidic central domain.
    • 翻译后修饰

      May be inactivated by phosphorylation on Ser-199 or Ser-204 (By similarity). Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA.
    • 细胞定位

      Nucleus. Nucleus speckle. Primarily, but not exclusively, nuclear and Cytoplasm. Found mainly in the cytoplasm.
    • Target information above from: UniProt accession Q12972 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 282319 Cow
      • Entrez Gene: 5511 Human
      • Entrez Gene: 100336 Mouse
      • Entrez Gene: 313030 Rat
      • Omim: 602636 Human
      • SwissProt: Q28147 Cow
      • SwissProt: Q12972 Human
      • SwissProt: Q8R3G1 Mouse
      • Unigene: 533474 Human
      see all
    • 别名

      • Activator of RNA decay antibody
      • ARD 1 antibody
      • ARD-1 antibody
      • ARD1 antibody
      • Homolog of E.coli RNase E antibody
      • NIPP 1 antibody
      • NIPP-1 antibody
      • NIPP1 antibody
      • Nuclear inhibitor of protein phosphatase 1 antibody
      • nuclear inhibitor of protein phosphatase-1 alpha antibody
      • Nuclear subunit of PP1 antibody
      • PP1R8_HUMAN antibody
      • PPP1R8 antibody
      • PRO2047 antibody
      • Protein phosphatase 1 regulatory inhibitor subunit 8 antibody
      • Protein phosphatase 1 regulatory subunit 8 antibody
      • RNase E antibody
      • RNase E, E. coli, homolog of antibody
      see all

    图片

    • Western blot - Anti-NIPP1 antibody (ab5300)
      Western blot - Anti-NIPP1 antibody (ab5300)
      All lanes : Anti-NIPP1 antibody (ab5300) at 1 µg/ml

      Lane 1 : HeLa nuclear lysate in RIPA buffer
      Lane 2 : HepG2 nuclear lysate in RIPA buffer
      Lane 3 : Jurkat nuclear lysate in RIPA buffer
      Lane 4 : NIH3T3 nuclear lysate in RIPA buffer

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 40 kDa



      Primary incubation for 1 hour. Detected using chemiluminescence.

    实验方案

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (1)

    发表研究结果有使用 ab5300?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5300 被引用在 1 文献中.

    • Nuytten M  et al. The transcriptional repressor NIPP1 is an essential player in EZH2-mediated gene silencing. Oncogene 27:1449-60 (2008). WB . PubMed: 17724462

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