Anti-NIPP1抗体(ab5300)
Key features and details
- Goat polyclonal to NIPP1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-NIPP1抗体
参阅全部 NIPP1 一抗 -
描述
山羊多克隆抗体to NIPP1 -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Dog
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免疫原
Synthetic peptide corresponding to Human NIPP1 aa 114-127 (C terminal).
Sequence:C-EAWPGKKPTPSLLI
(Peptide available asab23096) -
阳性对照
- WB: HeLa, HepG2, Jurkat and NIH3T3 nuclear lysates. IHC-P: Human thymus tissue.
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常规说明
This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody说明
This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 2.5 - 3.8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
IHC-P
Use a concentration of 2.5 - 3.8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.
Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing. -
组织特异性
Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells. -
序列相似性
Contains 1 FHA domain. -
结构域
Has a basic N- and C-terminal and an acidic central domain. -
翻译后修饰
May be inactivated by phosphorylation on Ser-199 or Ser-204 (By similarity). Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA. -
细胞定位
Nucleus. Nucleus speckle. Primarily, but not exclusively, nuclear and Cytoplasm. Found mainly in the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 282319 Cow
- Entrez Gene: 5511 Human
- Entrez Gene: 100336 Mouse
- Entrez Gene: 313030 Rat
- Omim: 602636 Human
- SwissProt: Q28147 Cow
- SwissProt: Q12972 Human
- SwissProt: Q8R3G1 Mouse
see all -
别名
- Activator of RNA decay antibody
- ARD 1 antibody
- ARD-1 antibody
see all
图片
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Western blot - Anti-NIPP1 antibody (ab5300)All lanes : Anti-NIPP1 antibody (ab5300) at 1 µg/ml
Lane 1 : HeLa nuclear lysate in RIPA buffer
Lane 2 : HepG2 nuclear lysate in RIPA buffer
Lane 3 : Jurkat nuclear lysate in RIPA buffer
Lane 4 : NIH3T3 nuclear lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 40 kDaPrimary incubation for 1 hour. Detected using chemiluminescence.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab5300 被引用在 1 文献中.
- Nuytten M et al. The transcriptional repressor NIPP1 is an essential player in EZH2-mediated gene silencing. Oncogene 27:1449-60 (2008). WB . PubMed: 17724462
