Anti-NIPA1抗体(ab121744)
Key features and details
- Rabbit polyclonal to NIPA1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NIPA1抗体 -
描述
兔多克隆抗体to NIPA1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant Protein Epitope Signature Tag, corresponding to amino acids 183-226 (HGPTNIMVYI SICSLLGSFT VPSTKGIGLA AQDILHNNPS SQRA) of Human NIPA1.
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阳性对照
- Human urinary bladder tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121744于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+). -
组织特异性
Widely expressed with highest levels in neuronal tissues. -
疾病相关
Defects in NIPA1 are the cause of spastic paraplegia autosomal dominant type 6 (SPG6) [MIM:600363]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. -
序列相似性
Belongs to the NIPA family. -
细胞定位
Cell membrane. Early endosome. Recruited to the cell membrane in response to low extracellular magnesium. - Information by UniProt
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数据库链接
- Entrez Gene: 123606 Human
- Omim: 608145 Human
- SwissProt: Q7RTP0 Human
- Unigene: 511797 Human
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别名
- FSP 3 antibody
- FSP3 antibody
- Magnesium transporter NIPA1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-NIPA1 antibody (ab121744)Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclei but not nucleoli. Recommended concentration of ab121744 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIPA1 antibody (ab121744)ab121744, at a 1/10 dilution, staining NIPA1 in paraffin-embedded Human urinary bladder tissue by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab121744 尚未被引用在任何文献中。