Anti-NINL抗体(ab121110)
Key features and details
- Rabbit polyclonal to NINL
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NINL抗体 -
描述
兔多克隆抗体to NINL -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to amino acids 682-787 of Human NINL.
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阳性对照
- Human cerebellum tissue. ICC/IF: U2-OS cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121110于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/0.25 - 1/2.
Fixation/Permeabilization: PFA/Triton X-100
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
1/0.25 - 1/2. Fixation/Permeabilization: PFA/Triton X-100
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Involved in the microtubule organization in interphase cells. Overexpression induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery; it also interferes with mitotic spindle assembly. May play a role in ovarian carcinogenesis. -
组织特异性
Expressed in KYSE150 esophageal carcinoma, HeLa cervical carcinoma and U2OS osteosarcoma cells. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase (at protein level). Expressed in fetal heart, skeletal muscle, liver, lung and cochlea, and in adult brain, testis, kidney and retina. -
序列相似性
Contains 4 EF-hand domains. -
结构域
The KEN and D (destructive) boxes are required for the cell cycle-controlled NINL degradation by the APC/C pathway. -
翻译后修饰
Phosphorylated by PLK1 which disrupts its centrosome association and interaction with gamma-tubulin.
Ubiquitinated by the APC/C complex leading to its degradation. -
细胞定位
Cytoplasm > cytoskeleton > centrosome. Cytoplasm. In interphase cells, NINL is transported to the centrosomes by the dynein-dynactin motor complex. During centrosome maturation, PLK1 directly phosphorylates NINL resulting in its release into the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 22981 Human
- Omim: 609580 Human
- SwissProt: Q9Y2I6 Human
- Unigene: 631508 Human
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别名
- 4930519N13Rik antibody
- dJ691N24.1 antibody
- Gm1004, antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NINL antibody (ab121110)ab121101 at 1/100 dilution staining NINL in Paraffin-embedded Human Cerebellum tissue by Immunohistochemistry.
Note: moderate cytoplasmic positivity in purkinje cells. -
Immunocytochemistry/ Immunofluorescence - Anti-NINL antibody (ab121110)Immunocytochemistry/ Immunofluorescence analysis of U-2 OS labeling NINL with ab121110. The image shows localization to cytosol & cytokinetic bridge.Fixation/Permeabilization: PFA/Triton X-100
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab121110 被引用在 1 文献中.
- Yin L et al. Epidermal gene expression and ethnic pigmentation variations among individuals of Asian, European and African ancestry. Exp Dermatol 23:731-5 (2014). IHC-P ; Human . PubMed: 25055985