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使用敲除细胞株进行验证重组RabMAb

重组Anti-Niemann Pick C2抗体[EPR19993] - Low endotoxin，Azide free (ab223550)

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Western blot - Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)
  • Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19993] to Niemann Pick C2 - Low endotoxin, Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

概述

  • 产品名称

    Anti-Niemann Pick C2抗体[EPR19993] - Low endotoxin，Azide free
    参阅全部 Niemann Pick C2 一抗

  • 描述

    兔单克隆抗体[EPR19993] to Niemann Pick C2 - Low endotoxin，Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Human fetal kidney and spleen lysates; HEK-293T, HepG2, C6, RAW 264,7, PC-12 and NIH/3T3 whole cell lysates; mouse spleen lysate; rat kidney lysate. IHC-P: Human endometrium and endometrium cancer tissues; mouse testis tissue; rat kidney tissue.

  • 常规说明

    ab223550 is the carrier-free version of ab207158.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab223550于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ab93684 can be used for antigen retrieval buffer.
说明
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ab93684 can be used for antigen retrieval buffer.

靶标

  • 功能

    May be involved in the regulation of the lipid composition of sperm membranes during the maturation in the epididymis.

  • 组织特异性

    Epididymis.

  • 疾病相关

    Defects in NPC2 are the cause of Niemann-Pick disease type C2 (NPDC2) [MIM:607625]. A lysosomal storage disorder that affects the viscera and the central nervous system. It is due to defective intracellular processing and transport of low-density lipoprotein derived cholesterol. It causes accumulation of cholesterol in lysosomes, with delayed induction of cholesterol homeostatic reactions. Niemann-Pick disease type C2 has a highly variable clinical phenotype. Clinical features include variable hepatosplenomegaly and severe progressive neurological dysfunction such as ataxia, dystonia and dementia. The age of onset can vary from infancy to late adulthood.

  • 序列相似性

    Belongs to the NPC2 family.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • EDDM1 antibody
      • Epididymal protein 1 antibody
      • Epididymal secretory protein antibody
      • Epididymal secretory protein E1 antibody
      • hE1 antibody
      • Human epididymis-specific protein 1 antibody
      • Niemann-Pick disease type C2 antibody
      • Niemann-Pick disease type C2 protein antibody
      • NPC intracellular cholesterol transporter 2 antibody
      • NPC2 antibody
      • NPC2_HUMAN antibody
      • Tissue specific secretory protein antibody
      see all

    图片

    • Western blot - Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)
      Western blot - Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)
      Lanes 1-2 : Anti-Niemann Pick C2 antibody [EPR19993] (ab207158) at 1/1000 dilution
      Lane 3 : Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550) at 1/1000 dilution

      Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : NPC2 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Observed band size: 16-18 kDa why is the actual band size different from the predicted?



      This data was developed using ab207158, the same antibody clone in a different buffer formulation.

      Lanes 1-3: Merged signal (red and green). Green - ab207158 observed at 16-18 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab207158 Anti-Niemann Pick C2 antibody [EPR19993] was shown to specifically react with Niemann Pick C2 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266749 (knockout cell lysate ab258079) was used. Wild-type and Niemann Pick C2 knockout samples were subjected to SDS-PAGE. ab207158 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)

      This IHC data was generated with the same anti-Niemann Pick C2 antibody clone [EPR19993] in a different buffer formulation (cat# ab207158).

      Immunohistochemical analysis of paraffin-embedded human endometrium cancer tissue labeling Niemann Pick C2 with ab207158 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human endometrium cancer is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)

      Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling Niemann Pick C2 with ab207158 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human endometrium is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207158).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)

      Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Niemann Pick C2 with ab207158 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse testis is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207158).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)

      Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Niemann Pick C2 with ab207158 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.  Cytoplasmic staining on rat kidney is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207158).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)
      Anti-Niemann Pick C2 antibody [EPR19993] - Low endotoxin, Azide free (ab223550)

    文献 (0)

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