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Neuroscience Neurotransmission Receptors / Channels Ligand-Gated Ion Channels nAch Receptors

Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4抗体(ab41172)

  • Datasheet
Reviews (1)Q&A (3)References (5)

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Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (ab41172)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (ab41172)

Key features and details

  • Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 4/CHRNA4
  • Suitable for: ELISA, ICC/IF, IHC-P, WB, IHC-Fr
  • Reacts with: Mouse, Rat, Chicken, Human, Chimpanzee, Pufferfish, Zebrafish, Snail
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4抗体
    参阅全部 Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 一抗
  • 描述

    兔多克隆抗体to Nicotinic Acetylcholine Receptor alpha 4/CHRNA4
  • 宿主

    Rabbit
  • 特异性

    Antiserum Specificity Polypeptide % Cross Reactivity Nicotinic a4 receptor (620-627) 100 Nicotinic a4 receptor ~80 Nicotinic a3 receptor 0 Nicotinic a5 receptor 0 Nicotinic a7 receptor 0 Nicotinic ß2 receptor 0 Nicotinic ß3 receptor 0 Nicotinic ß4 receptor 0
  • 经测试应用

    适用于: ELISA, ICC/IF, IHC-P, WB, IHC-Frmore details
  • 种属反应性

    与反应: Mouse, Rat, Chicken, Human, Chimpanzee, Pufferfish, Zebrafish, Snail
  • 免疫原

    Synthetic peptide corresponding to Human Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 aa 620-627 (C terminal) conjugated to keyhole limpet haemocyanin (Cysteine residue).
    Sequence:

    PPWLAGMI


    (Peptide available as ab49523, ab99319)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • nAch Receptors
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab41172于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
1/250.
WB
1/800. Predicted molecular weight: 70 kDa. With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
IHC-Fr (1)
Use at an assay dependent concentration.
说明
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
1/250.
WB
1/800. Predicted molecular weight: 70 kDa. With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
IHC-Fr
Use at an assay dependent concentration.

靶标

  • 功能

    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • 疾病相关

    Defects in CHRNA4 are the cause of nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
  • 序列相似性

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily.
  • 细胞定位

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Target information above from: UniProt accession P43681 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 395606 Chicken
    • Entrez Gene: 1137 Human
    • Entrez Gene: 11438 Mouse
    • Entrez Gene: 25590 Rat
    • Omim: 118504 Human
    • SwissProt: P09482 Chicken
    • SwissProt: P43681 Human
    • SwissProt: O70174 Mouse
    • SwissProt: P09483 Rat
    • Unigene: 10734 Human
    • Unigene: 252369 Mouse
    • Unigene: 9697 Rat
    see all
  • 别名

    • A4 nicotinic receptor antibody
    • Acetylcholine receptor alpha 4 neural antibody
    • Acetylcholine receptor neuronal nicotinic alpha 4 subunit antibody
    • ACH 4 antibody
    • ACH4 antibody
    • ACHA4_HUMAN antibody
    • AChR antibody
    • Acra 4 antibody
    • Acra4 antibody
    • Alpha4 nAChR antibody
    • BFNC antibody
    • Cholinergic receptor nicotinic alpha 4 antibody
    • Cholinergic receptor nicotinic alpha polypeptide 4 antibody
    • CHRNA 4 antibody
    • CHRNA4 antibody
    • EBN 1 antibody
    • EBN antibody
    • EBN1 antibody
    • NACHR antibody
    • NACRA 4 antibody
    • NACRA4 antibody
    • Neuronal acetylcholine receptor subunit alpha-4 antibody
    • Neuronal nicotinic acetylcholine receptor alpha 4 subunit antibody
    see all

图片

  • Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (ab41172)
    Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (ab41172)
    All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (ab41172) at 1/800 dilution

    Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (ab41172)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (ab41172)

    ab41172 (1/250 dilution) staining Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear and membrane compartment within the glia region of cells
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

实验方案

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (5)

发表研究结果有使用 ab41172?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab41172 被引用在 5 文献中.

  • Wang Q  et al. Nicotine Activating α4β2 Nicotinic Acetylcholine Receptors to Suppress Neuroinflammation via JAK2-STAT3 Signaling Pathway in Ischemic Rats and Inflammatory Cells. Mol Neurobiol 59:3280-3293 (2022). PubMed: 35297011
  • Seoane-Collazo P  et al. Central nicotine induces browning through hypothalamic ? opioid receptor. Nat Commun 10:4037 (2019). PubMed: 31492869
  • Montaño-Velázquez BB  et al. Influence of Tobacco Smoke Exposure on the Protein Expression of a7 and a4 Nicotinic Acetylcholine Receptors in Squamous Cell Carcinoma Tumors of the Upper Aerodigestive Tract (Out of the Larynx). Subst Abuse 12:1178221818801316 (2018). PubMed: 31068752
  • Chen N  et al. An acetylcholine-activated microcircuit drives temporal dynamics of cortical activity. Nat Neurosci 18:892-902 (2015). IHC . PubMed: 25915477
  • Pubill D  et al. 3,4-Methylenedioxy-methamphetamine induces in vivo regional up-regulation of central nicotinic receptors in rats and potentiates the regulatory effects of nicotine on these receptors. Neurotoxicology 35:41-9 (2013). PubMed: 23261423

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1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 24°C
Sample
Snail (Helix pomatia) Tissue sections (brain)
Specification
brain
Permeabilization
Yes - TX
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 27 2013

Question

Dear xxxx,

As far as I know, unfortunately there is no data about the protein sequences of the Helix pomatia acetylcholine receptor alpha 4 and 7. Even so I hope I can test one of the antibodies.

Best regards,

Read More

Abcam community

Verified customer

Asked on Nov 12 2012

Answer

Thank you for your reply.

As we have no sequence for the proteins from Helix pomatia I cannot predict how likely the antibodies you would like to try will cross react. We would therefore not usually offer the testing discount scheme for this. However I am willing to make an exception in this case if you would still like to try it. The testing discount codes for the two antibodies as follows:

ab10096, discount code: xxxxxx, expiration date: 13th March 2013, value: €380.00

ab41172, discount code: xxxxxx, expiration date: 13th March 2013, value: €370.00

The codes above will give you €380.00 and €370.00 respectively off your next order before the expiration date. To redeem this offer, please submit an Abreview for Helix pomatia in Western blotting and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site:

www.abcam.com/Abreviews

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here:

www.abcam.com/abtrial

Read More

Abcam Scientific Support

回复于 Nov 12 2012

Question

Thanks a lot for your answer it was really fast!
We do agree to order and get discount for the antibodies you have suggested:

CDC42 Binding Protein Kinase Beta antibody ab61328

CNKR2 antibody ab64844

NEK7 antibody ab80948

MEK3 antibody ab5428

BMX antibody ab73887

Muscarinic Acetylcholine Receptor 1 antibody ab75178

PPP4C antibody sb70888
But I have one more question.
On the https://www.abcam.com/index.html?pageconfig=resource&rid=11998 of your site in FAQ part, the last question isreferred to the antibodies that have already been tested in the applications for some species, but have no image. And the answer says that there is a possibility to get discount also for those antibodies.
There are still 11 antibodies that have no images for the proposed species. if it is possible to give us some more discounts, we will submit the reviews including images for the proposed antibodies.
Can you please have a look one more time?

Thank you!
Best regards

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Abcam community

Verified customer

Asked on Mar 05 2012

Answer

Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these additional two antibodies:
ab13898
ab28669
I hope this will be helpful. I look forward to receiving your confirmation that you would like to go ahead, and I will be pleased to arrange and send the discount codes to you.
For the remaining antibodies, I can suggest you may like to consider submitting an Abreview anyway, for which you can obtain Abpoints:
ab85612
ab75874
ab61113
ab47504
ab16476
ab85608
ab31232
ab41172
ab23832
Thank you for your time and continued interest in our products. I look forward to hearing from you.

Read More

Abcam Scientific Support

回复于 Mar 05 2012

Question

Dear support team,
We kindly appreciate the quality of your products and technical support!

According to the rules of your 100% Testing Discount we would like to test a number of primary antibodies in Western blotting for Native and reduced conditions. The list of antibodies with catalog numbers and species is attached.

As a permanent client of your company and active research team we will provide you with reliable reviews in short terms.
Hope for a good cooperation.






Best regards

Read More

Abcam community

Verified customer

Asked on Mar 02 2012

Answer

Thank you very much for your interest in our antibodies.

We appreciate receiving feedback and data from customers, and we do have a testing discount offer available.

If a customer would like to test an antibody in an application or species that it has not been previously tested in, we are able to provide our testing discount program.

In this case, the antibodiess you have listed have all been tested in western blot already, but we would be able to provide this offer to you to test the following antibodies in mouse and rat:

Discount to detect in mouse and rat (the following antibodies have not yet been tested in mouse or rat):
CDC42 Binding Protein Kinase Beta antibody ab61328
CNKR2 antibody ab64844
NEK7 antibody ab80948

Discount to test in mouse (the following antibodies have not yet been tested in mouse):
MEK3 antibody ab5428
BMX antibody ab73887
Muscarinic Acetylcholine Receptor 1 antibody ab75178

Discount to test in rat (the following antibodies have not been tested in rat):
PPP4C antibody sb70888

The following are already tested in western blot, and in mouse and/or rat as per your list. I am sorry we are therefore not able to provide a testing discount for these.
ROCK2 antibody ab85612 Already tested WB, ms and rat
CAMKIV antibody [EP2564Y] ab75874 Already tested WB, ms and rat
FAK antibody ab61113 Already tested WB, ms and rat
S6K antibody ab47504 Previously tested WB, ms and rat
PPP1A antibody ab 16476 Previously tested WB, ms and rat
5HT7 Receptor antibody ab13898 WB and mouse already tested.
NMDAR1 antibody ab28669 Previously tested WB, ms and rat
Dopamine Receptor D1 antibody ab85608 Previously tested WB, ms and rat
Glutamate Receptor 1 (AMPA subtype) antibody ab31232 Previously tested WB and rat
Nicotinic Acetylcholine Receptor alpha 4 antibody ab41172 Previously tested WB and mouse
Nicotinic Acetylcholine Receptor alpha 7 antibody ab23832 Previously tested WB and rat

For the antibodies we can provide a discount for, I can offer a discount off a future purchase if you buy the antibody(ies) now, test them in mouse, rat or both as indicated above, and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount provided for each individual antibody would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test these antibodies in mouse, rat or both. I will then send a discount code. This code must be issued before purchasing the antibodies so please wait for my reply before ordering.

2. Purchase the antibodies either by phone, fax, or online (www.abcam.com).

3. Test it in mouse, rat or both.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any [PRIMARY ANTIBODY /PROTEIN] ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for the species, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Also, we are able to provide a discount for any order of 5 items or more. Therefore, once you have decided which antibodies you are going to purchase, please contact our customer service department before you place the order with a full list of the antibodies you wish to buy (orders@abcam.com). They will then be able to provide you with a quote.

I hope this will be helpful to you. I look forward to receiving confirmation that you would like to receive the discount codes. If you have any further questions, please do not hesitate to contact me.

Read More

Abcam Scientific Support

回复于 Mar 02 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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