Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4抗体(ab41172)
Key features and details
- Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 4/CHRNA4
- Suitable for: ELISA, ICC/IF, IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat, Chicken, Human, Chimpanzee, Pufferfish, Zebrafish, Snail
- Isotype: IgG
概述
-
产品名称
Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4抗体
参阅全部 Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 一抗 -
描述
兔多克隆抗体to Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 -
宿主
Rabbit -
特异性
Antiserum Specificity Polypeptide % Cross Reactivity Nicotinic a4 receptor (620-627) 100 Nicotinic a4 receptor ~80 Nicotinic a3 receptor 0 Nicotinic a5 receptor 0 Nicotinic a7 receptor 0 Nicotinic ß2 receptor 0 Nicotinic ß3 receptor 0 Nicotinic ß4 receptor 0 -
经测试应用
适用于: ELISA, ICC/IF, IHC-P, WB, IHC-Frmore details -
种属反应性
与反应: Mouse, Rat, Chicken, Human, Chimpanzee, Pufferfish, Zebrafish, Snail -
免疫原
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituents: PBS, 1% BSA -
Concentration information loading...
-
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab41172于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
|
IHC-P |
1/250.
|
|
WB |
1/800. Predicted molecular weight: 70 kDa. With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
|
|
IHC-Fr | (1) |
Use at an assay dependent concentration.
|
说明 |
---|
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
1/250. |
WB
1/800. Predicted molecular weight: 70 kDa. With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk. |
IHC-Fr
Use at an assay dependent concentration. |
靶标
-
功能
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. -
疾病相关
Defects in CHRNA4 are the cause of nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements. -
序列相似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily. -
细胞定位
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 395606 Chicken
- Entrez Gene: 1137 Human
- Entrez Gene: 11438 Mouse
- Entrez Gene: 25590 Rat
- Omim: 118504 Human
- SwissProt: P09482 Chicken
- SwissProt: P43681 Human
- SwissProt: O70174 Mouse
see all -
别名
- A4 nicotinic receptor antibody
- Acetylcholine receptor alpha 4 neural antibody
- Acetylcholine receptor neuronal nicotinic alpha 4 subunit antibody
see all
图片
-
All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (ab41172) at 1/800 dilution
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 70 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (ab41172)
ab41172 (1/250 dilution) staining Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear and membrane compartment within the glia region of cells
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
数据表及文件
-
Datasheet download
文献 (5)
ab41172 被引用在 5 文献中.
- Wang Q et al. Nicotine Activating α4β2 Nicotinic Acetylcholine Receptors to Suppress Neuroinflammation via JAK2-STAT3 Signaling Pathway in Ischemic Rats and Inflammatory Cells. Mol Neurobiol 59:3280-3293 (2022). PubMed: 35297011
- Seoane-Collazo P et al. Central nicotine induces browning through hypothalamic ? opioid receptor. Nat Commun 10:4037 (2019). PubMed: 31492869
- Montaño-Velázquez BB et al. Influence of Tobacco Smoke Exposure on the Protein Expression of a7 and a4 Nicotinic Acetylcholine Receptors in Squamous Cell Carcinoma Tumors of the Upper Aerodigestive Tract (Out of the Larynx). Subst Abuse 12:1178221818801316 (2018). PubMed: 31068752
- Chen N et al. An acetylcholine-activated microcircuit drives temporal dynamics of cortical activity. Nat Neurosci 18:892-902 (2015). IHC . PubMed: 25915477
- Pubill D et al. 3,4-Methylenedioxy-methamphetamine induces in vivo regional up-regulation of central nicotinic receptors in rats and potentiates the regulatory effects of nicotine on these receptors. Neurotoxicology 35:41-9 (2013). PubMed: 23261423