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Anti-Nicastrin抗体(ab3444)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (4)

Product price, shipping and contact information

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Western blot - Anti-Nicastrin antibody (ab3444)
  • Western blot - Anti-Nicastrin antibody (ab3444)
  • Western blot - Anti-Nicastrin antibody (ab3444)

Key features and details

  • Rabbit polyclonal to Nicastrin
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Nicastrin antibody [EPR2575Y] (ab68145)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Nicastrin抗体
    参阅全部 Nicastrin 一抗
  • 描述

    兔多克隆抗体to Nicastrin
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Nicastrin aa 650-750.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: T98G, RSC96, mouse neuronal cells, HeLa, IMR-32, NIH/3T3, L6, mouse brain, rat brain
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer’s disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE). This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Found associated with the presenilins is the transmembrane glycoprotein nicastrin. Nicastrin has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Also found in the neurofibrillary lesions associated with Alzheimer’s disease is a protein termed Tau. Tau is a neuronal microtubule-associated protein found predominantly on axons. Tau functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major constituent of paired helical filaments (PHF), which are the building block of neurofibrillary lesions found in brain tissue of Alzheimer’s diseased patients.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Proteases
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Cytoplasmic

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3444于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 78 kDa).
说明
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 78 kDa).

靶标

  • 功能

    Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.
  • 组织特异性

    Widely expressed.
  • 序列相似性

    Belongs to the nicastrin family.
  • 细胞定位

    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession Q92542 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 23385 Human
    • Entrez Gene: 59287 Mouse
    • Entrez Gene: 289231 Rat
    • Omim: 605254 Human
    • SwissProt: Q92542 Human
    • SwissProt: P57716 Mouse
    • SwissProt: Q8CGU6 Rat
    • Unigene: 517249 Human
    • Unigene: 218203 Mouse
    • Unigene: 51975 Rat
    see all
  • 别名

    • Anterior pharynx defective 2 antibody
    • APH 2 antibody
    • APH2 antibody
    • ATAG1874 antibody
    • KIAA0253 antibody
    • Ncstn antibody
    • NCT antibody
    • NICA_HUMAN antibody
    • Nicastrin antibody
    • RP11 517F10.1 antibody
    • RP11517F101 antibody
    see all

图片

  • Western blot - Anti-Nicastrin antibody (ab3444)
    Western blot - Anti-Nicastrin antibody (ab3444)
    All lanes : Anti-Nicastrin antibody (ab3444) at 1 µg/ml

    Lane 1 : HeLa whole cell extract
    Lane 2 : IMR-32 whole cell extract (human neuroblastoma cell line)
    Lane 3 : NIH/3T3 whole cell extract (mouse embryo fibroblast cell line)
    Lane 4 : L6 whole cell extract (Rat skeletal muscle cell line)
    Lane 5 : mouse brain
    Lane 6 : rat brain

    Lysates/proteins at 60 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 78 kDa

  • Western blot - Anti-Nicastrin antibody (ab3444)
    Western blot - Anti-Nicastrin antibody (ab3444)
    All lanes : Anti-Nicastrin antibody (ab3444) at 2 µg/ml

    Lane 1 : T98G membrane extract (Human glioblastoma cell line)
    Lane 2 : RSC96 membrane extract (spontaneously immortalized rat Schwann cell line )

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 78 kDa

  • Western blot - Anti-Nicastrin antibody (ab3444)
    Western blot - Anti-Nicastrin antibody (ab3444)

    Western blot detection of Nicastrin on mouse primary cultured neuronal cells using ab3444. 

实验方案

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (4)

发表研究结果有使用 ab3444?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3444 被引用在 4 文献中.

  • Liu Y  et al. Plasmalogen attenuates the development of hepatic steatosis and cognitive deficit through mechanism involving p75NTR inhibition. Redox Biol 43:102002 (2021). PubMed: 33984602
  • Pintchovski SA  et al. Evidence that enzyme processivity mediates differential Aß production by PS1 and PS2. Curr Alzheimer Res 10:4-10 (2013). WB . PubMed: 22950863
  • Steuble M  et al. Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport. Biol Open 1:761-74 (2012). PubMed: 23213470
  • Massey LK  et al. Ubiquilin regulates presenilin endoproteolysis and modulates gamma-secretase components, Pen-2 and nicastrin. Biochem J 391:513-25 (2005). PubMed: 15975090

客户评价及客户问答

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Question

I work with with Dictyostelium discoideum which has a diverged Nicastrin orthologue. I am interested in seeing ahead of time if the sequence in which any of your Nicastrin antibodies is against is somewhat conserved in my organism before I buy one to find out whether it is worth trying or not. If I send you the sequence, can you check this for me to see if the region where the antibody is against is conserved? I have pasted the sequence below: MYTSLNSYPCTRIMTLNGQIGCSSSHGGDSGILYLIDSDESYHNYFSYNQQKDIIVVFDS NYFNKTLVLEMYSKKKMNGALVLTDIGKTYPYSPEDQYPIKQFGLYPDSNLNWNPNGDGF TYMNFPFPMFALELKTSIIIRNLSTINRDGKYPAYGAELDSFMQGAINAETCLRRGFCEP VGGQSIWSSFSEVIDQSKPIILVMLPIDATAFFRDLATGTDQSGYALTVLLSMLNTLQGV DKTKWDKEVIFAMWNSERWGYVGSTNFVNDLLNFNCTSLDSNNQNSCSSPPMLDLTFEQI KFENIYAIIEFNQIGRPVNSGKKTPNKLDIYNLVFHPNGGAGANQLMDVFSQSTQSYENS TIQFQKTTQNELPPCSSMSFIKEINKKSAPNFIGTLVITDHDYQYNNPYFGDEQDNSGNI NTTTSTLFDMVQVFSKSIDLLAGGNGTVKVDDLFIREINVCLTQSITCNWVTKLMSTFPY NPIPNFYSGVYGVSPVNHITPIETRFIFRMATYLTQHRTNATNCTSDNDCDTSSSICVNK VCLYSNTHYHNAISLAFSFDNSKSSWTIVNTSYPVFVESNWDYTTVRLFQVGSYANEIWF LVSGLIELLLSVGIIFYIKKYLSKRYKLL*

Read More

Abcam community

Verified customer

Asked on Jan 30 2007

Answer

Thank you for your patience. I have received a reply from the originator of ab45425 (Nicastrin antibody). The immunogenic peptide sequence is proprietary information but they have performed a sequence alignment for you. Based on the alignment results, we know it is unlikely that this product will react with Dictyostelium discoideum and we do not recommend using ab45425 with this species. Please don't hesitate to contact us again if you have further questions.

Read More

Abcam Scientific Support

回复于 Feb 01 2007

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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