重组Anti-NHP2抗体[EPR12404] (ab180498)
![Western blot - Anti-NHP2 antibody [EPR12404] (ab180498)](https://www.abcam.cn/ps/products/180/ab180498/Images/ab180498-211141-anti-nhp2-antibody-epr12404-western-blot.jpg "Western blot - Anti-NHP2 antibody [EPR12404] (ab180498)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12404] to NHP2
- Suitable for: Flow Cyt (Intra), IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-NHP2抗体[EPR12404]
参阅全部 NHP2 一抗 -
描述
兔单克隆抗体[EPR12404] to NHP2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human NHP2 aa 1-100. The exact sequence is proprietary.
Database link: Q9NX24 -
阳性对照
- K562, Jurkat, Caco 2 and SH-SY5Y lysates; Human ovarian carcinoma tissue; K562 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12404 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180498于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). |
靶标
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功能
Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. -
组织特异性
Expressed in brain, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen, testis and thymus. Also expressed at lower levels in the liver. -
疾病相关
Dyskeratosis congenita, autosomal recessive, 2 -
序列相似性
Belongs to the ribosomal protein L7Ae family. -
发展阶段
Transcript peaks at G1/S transition. -
细胞定位
Nucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies. - Information by UniProt
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数据库链接
- Entrez Gene: 55651 Human
- Entrez Gene: 52530 Mouse
- Entrez Gene: 287273 Rat
- Omim: 606470 Human
- SwissProt: Q9NX24 Human
- SwissProt: Q9CRB2 Mouse
- Unigene: 744074 Human
- Unigene: 28203 Mouse
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别名
- DKCB2 antibody
- FLJ20479 antibody
- H/ACA ribonucleoprotein complex subunit 2 antibody
see all
图片
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Western blot - Anti-NHP2 antibody [EPR12404] (ab180498)All lanes : Anti-NHP2 antibody [EPR12404] (ab180498) at 1/2000 dilution
Lane 1 : K562 lysate
Lane 2 : Jurkat lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2 antibody [EPR12404] (ab180498)](https://www.abcam.cn/ps/products/180/ab180498/Images/ab180498-211144-anti-nhp2-antibody-epr12404-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2 antibody [EPR12404] (ab180498)Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling NHP2 with ab180498 at 1/500 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG and counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-NHP2 antibody [EPR12404] (ab180498)](https://www.abcam.cn/ps/products/180/ab180498/Images/ab180498-211146-anti-nhp2-antibody-epr12404-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-NHP2 antibody [EPR12404] (ab180498)Intracellular flow cytometric analysis of K562 cells (2% paraformaldehyde-fixed)labeling NHP2 with ab180498 at 1/20 (red) compared to a Rabbit monoclonal IgGnegative control antibody (green) followed byGoat anti rabbit IgG (FITC) at 1/75 dilution.
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![Anti-NHP2 antibody [EPR12404] (ab180498)](https://www.abcam.cn/ps/products/180/ab180498/Images/ab180498-4-benefits-of-recombinant-antibodies.png)
Anti-NHP2 antibody [EPR12404] (ab180498)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (5)
ab180498 被引用在 5 文献中.
- Gong Y et al. Age-Associated Proteomic Signatures and Potential Clinically Actionable Targets of Colorectal Cancer. Mol Cell Proteomics 20:100115 (2021). PubMed: 34129943
- Ryu H et al. The deubiquitinase USP36 promotes snoRNP group SUMOylation and is essential for ribosome biogenesis. EMBO Rep 22:e50684 (2021). PubMed: 33852194
- Tang S et al. Knockdown of NHP2 inhibits hepatitis B virus X protein-induced hepatocarcinogenesis via repressing TERT expression and disrupting the stability of telomerase complex. Aging (Albany NY) 12:19365-19374 (2020). PubMed: 33044946
- Benyelles M et al. NHP2 deficiency impairs rRNA biogenesis and causes pulmonary fibrosis and Høyeraal-Hreidarsson syndrome. Hum Mol Genet 29:907-922 (2020). PubMed: 31985013
- Quan Wang J et al. Discovery of new chromen-4-one derivatives as telomerase inhibitors through regulating expression of dyskerin. J Enzyme Inhib Med Chem 33:1199-1211 (2018). PubMed: 30132373