AB174323

重组Anti-NgR3抗体[EPR11334]

Anti-NgR3 antibody [EPR11334]

RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal NgR3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

查看别名

NGRH2, NGRL2, RTN4RL1, Reticulon-4 receptor-like 1, Nogo receptor-like 2, Nogo-66 receptor homolog 2, Nogo-66 receptor-related protein 3, NgR3

2 Images

Flow Cytometry (Intracellular) - Anti-NgR3 antibody [EPR11334] (AB174323)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NgR3 antibody [EPR11334] (AB174323)

Intracellular flow cytometric analysis of permeabilized K562 cells labeling NgR3 with ab174323 at 1/100 dilution (red) compared with a rabbit IgG negative control (green).

Supplier Data

Western blot - Anti-NgR3 antibody [EPR11334] (AB174323)

All lanes:

Western blot - Anti-NgR3 antibody [EPR11334] (ab174323) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

MCF-7 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 49 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-NgR3 antibody [EPR11334] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11334

亚型

IgG

不含载体蛋白

反应种属

Human

应用

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NgR3 also known as Nogo-66 Receptor Homolog 3 or Reticulon 4 Receptor-Like 2 is part of the Nogo receptor family. It is a glycolipid-anchored receptor weighing approximately 55 kDa. This protein is mainly found in the central nervous system and is widely expressed in the brain though it also appears in other tissues to a lesser extent. NgR3 contributes to axon growth inhibition and neuronal regeneration influencing nerve repair processes.

Biological function summary

NgR3 influences axonal guidance and inhibition by interacting with other proteins. It forms part of a receptor complex typically incorporating p75 neurotrophin receptor and Lingo-1 which are important for signaling processes. Through these interactions NgR3 helps moderate the effects of myelin-associated inhibitors on axonal outgrowth supporting the regulation of neuronal plasticity and myelin repair mechanisms.

Pathways

This receptor plays a significant role in the regulation of axon regeneration. It intersects with the Nogo-A signaling pathway where it works with closely related proteins such as NgR1 and NgR2. These interactions inhibit axonal growth and impact neuronal development and recovery post-injury. Through this pathway NgR3 aids in balancing axonal regeneration and pathfinding during neural development and repair.

NgR3 is associated with conditions that involve axonal damage and neurodegeneration including multiple sclerosis and spinal cord injuries. It impacts these conditions by modulating axonal growth inhibitory mechanisms. In multiple sclerosis NgR3 works with proteins like Nogo-A to influence remyelination processes potentially affecting the disease progression. Understanding the role of NgR3 in these contexts could aid in developing therapeutic strategies aimed at enhancing neural repair and recovery.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Cell surface receptor. Plays a functionally redundant role in postnatal brain development and in regulating axon regeneration in the adult central nervous system. Contributes to normal axon migration across the brain midline and normal formation of the corpus callosum. Protects motoneurons against apoptosis; protection against apoptosis is probably mediated by MAG. Plays a role in inhibiting neurite outgrowth and axon regeneration via its binding to neuronal chondroitin sulfate proteoglycans. Binds heparin (By similarity). Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development (PubMed : 22325200). Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton (PubMed : 22325200).

See full target information RTN4RL1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 15:880382 PubMed35592113

2022

Stress Elicits Contrasting Effects on Rac1-Cofilin Signaling in the Hippocampus and Amygdala.

Applications

Unspecified application

Species

Unspecified reactive species

Mihika Bose,Mohammad Sarfaraz Nawaz,Rakhi Pal,Sumantra Chattarji

Saudi journal of biological sciences 28:4132-4136 PubMed34354392

2021

Effect of ganglioside combined with Chip Jiaji electro-acupuncture on Nogo-NgR signal pathway in SCI rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hongfeng Hu,Hui Wang,Wei Liu

View all publications

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重组Anti-NgR3抗体[EPR11334]