AB221609

重组Anti-proNGF抗体[EP1320Y] - Low endotoxin，Azide free

Anti-NGF antibody [EP1320Y] - Low endotoxin, Azide free

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Recombinant Monoclonal NGF antibody. Propeptide domain. Carrier free. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

查看别名

NGFB, NGF, Beta-nerve growth factor, Beta-NGF

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-NGF antibody [EP1320Y] - Low endotoxin, Azide free (AB221609)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NGF antibody [EP1320Y] - Low endotoxin, Azide free (AB221609)

Immunofluorescence staining of U87-MG cells with purified ab52918 at a working dilution of 1 in 300 counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor^® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor^® 488 goat anti rabbit used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control purified ab52918 was used at a dilution of 1/200 followed by an Alexa Fluor^® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA glycerol and sodium azide (ab52918).

不同偶联物与剂型 (2)

603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-NGF antibody [EP1320Y]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-NGF antibody [EP1320Y]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP1320Y

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "For unpurified, use 1/500.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "For unpurified, use 1/500.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "For unpurified, use 1/500.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

产品详情

ab221609 is the carrier-free version of ab52918.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

style

left-column

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed : 14976160, PubMed : 20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed : 20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed : 20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).

See full target information NGF

style

left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 56: PubMed41041859

2025

CENPU promotes tumorigenesis and stem cell properties in triple‑negative breast cancer by suppressing lysosomal furin degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Shujuan Sun,Zhenyu Hou,Ling Qiang,Dongdong Zhou

Biological & pharmaceutical bulletin 45:27-33 PubMed34980778

2022

Coadministration of Curcumin and Hydromorphone Hydrochloride Alleviates Postoperative Pain in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yihan Wang,Yang Liu,Jieting Liu,Min Wang,Yingbin Wang

Bone 151:116034 PubMed34107348

2021

Cartilage tissue miR-214-3p regulates the TrkB/ShcB pathway paracrine VEGF to promote endothelial cell migration and angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Xiao,Xu Zhu,Jinpeng Sun,Yuhang Zhang,Weijian Qiu,Jianqiang Li,Xuejian Wu

Folia neuropathologica 59:67-80 PubMed33969678

2021

MiR-1b up-regulation inhibits rat neuron proliferation and regeneration yet promotes apoptosis via targeting KLF7.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojie Li,Lihe Yuan,Jin Wang,Zun Zhang,Shaojing Fu,Shaobin Wang,Xinhui Li

PloS one 11:e0165586 PubMed27792755

2016

Blocking Nerve Growth Factor Signaling Reduces the Neural Invasion Potential of Pancreatic Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aditi A Bapat,Ruben M Munoz,Daniel D Von Hoff,Haiyong Han

Evidence-based complementary and alternative medic 2016:8403064 PubMed27800006

2016

The Effect of Repeated Electroacupuncture Analgesia on Neurotrophic and Cytokine Factors in Neuropathic Pain Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Junying Wang,Yonghui Gao,Shuping Chen,Chenlin Duanmu,Jianliang Zhang,Xiumei Feng,Yaxia Yan,Junling Liu,Gerhard Litscher

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-proNGF抗体[EP1320Y] - Low endotoxin，Azide free