Anti-NGDN抗体(ab121124)
Key features and details
- Rabbit polyclonal to NGDN
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NGDN抗体 -
描述
兔多克隆抗体to NGDN -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to amino acids 61-209 of Human NGDN (Q8NEJ9).
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阳性对照
- NGDN transfected HEK293T lysate, Human skin tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121124于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
靶标
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功能
Involved in the translational repression of cytoplasmic polyadenylation element (CPE)-containing mRNAs. -
序列相似性
Belongs to the SAS10 family. -
细胞定位
Nucleus. Cytoplasm. Cell projection > axon. Cell projection > dendrite. Cell projection > filopodium. Detected in axons, dendrites and filopodia. Colocalized with EIF4E in neurites. - Information by UniProt
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数据库链接
- Entrez Gene: 25983 Human
- Omim: 610777 Human
- SwissProt: Q8NEJ9 Human
- Unigene: 9043 Human
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别名
- C14orf120 antibody
- EIF4E-binding protein antibody
- Neuroguidin antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-NGDN antibody (ab121124)Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus, nucleoli & mitochondria.
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Western blot - Anti-NGDN antibody (ab121124)All lanes : Anti-NGDN antibody (ab121124) at 1/250 dilution
Lane 1 : vector only transfected HEK293T lysate
Lane 2 : NGDN-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Developed using the ECL technique.
10-20% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NGDN antibody (ab121124)ab121124 at 1/350 dilution staining NGDN in Paraffin-emebedded Human skin tissue by Immunohistochemistry. Note: strong nuclear positivity in epidermal cells.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab121124 尚未被引用在任何文献中。