Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-NG2抗体[EPR23752-147] - BSA and Azide free (ab275041)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)
  • Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)
  • Flow Cytometry - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)
  • Flow Cytometry - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)
  • Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)
  • Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23752-147] to NG2 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

PE Unconjugated

概述

  • 产品名称

    Anti-NG2抗体[EPR23752-147] - BSA and Azide free
    参阅全部 NG2 一抗

  • 描述

    兔单克隆抗体[EPR23752-147] to NG2 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, ICC/IF, Flow Cytmore details
    不适用于: IHC-Fr,IP or WB

  • 种属反应性

    与反应: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Mouse cerebrum tissue. ICC/IF: Mouse primary neural/glia cells; Rat primary neural/glia cells. Flow cyt: NIH/3T3 and mouse primary neural glia cells.

  • 常规说明

    ab275041 is the carrier-free version of ab259324.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab275041于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
应用说明
Is unsuitable for IHC-Fr,IP or WB.

靶标

  • 功能

    Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.

  • 组织特异性

    Detected only in malignant melanoma cells.

  • 序列相似性

    Contains 15 CSPG (NG2) repeats.
    Contains 2 laminin G-like domains.

  • 翻译后修饰

    O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
    Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.

  • 细胞定位

    Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 4732461B14Rik antibody
      • AN2 antibody
      • AN2 proteoglycan antibody
      • Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody
      • Chondroitin sulfate proteoglycan 4 antibody
      • Chondroitin sulfate proteoglycan NG2 antibody
      • Cspg4 antibody
      • Cspg4 chondroitin sulfate proteoglycan 4 antibody
      • CSPG4_HUMAN antibody
      • HMW-MAA antibody
      • HSN tumor-specific antigen antibody
      • Kiaa4232 antibody
      • MCSP antibody
      • MCSPG antibody
      • MEL-CSPG antibody
      • Melanoma chondroitin sulfate proteoglycan antibody
      • Melanoma-associated chondroitin sulfate proteoglycan antibody
      • MELCSPG antibody
      • MSK16 antibody
      • NG2 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)

      This data was developed using ab259324, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling NG2 with ab259324 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID: 31769737). The section was incubated with ab259324 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)
      Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)

      This data was developed using ab259324, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labelling NG2 with ab259324 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in mouse primary glia cells. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Flow Cytometry - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)
      Flow Cytometry - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)

      This data was developed using ab259324, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Mouse primary neural glia cells cells labelling NG2 with ab259324 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

      Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

    • Flow Cytometry - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)
      Flow Cytometry - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)

      This data was developed using ab259324, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of N9 (Mouse microglia, Left) / NIH/3T3 (Mouse embryonic fibroblast, Right) cells labelling NG2 with ab259324 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Negative control: N9 cells.

      Gated on viable cells.

    • Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)
      Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)

      This data was developed using ab259324, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cells labelling NG2 with ab259324 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in rat primary glia cells. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.  ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain MAP2 at 1/500 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      -ve control 1: ab259324 at 1/50 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

      -ve control 2: ab11267 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

    • Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)
      Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab275041？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab275041 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    There are currently no Customer reviews or Questions for ab275041.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com