Anti-NFX1抗体(ab176733)
Key features and details
- Rabbit polyclonal to NFX1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-NFX1抗体 -
描述
兔多克隆抗体to NFX1 -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human NFX1 aa 1070-1120. The exact sequence is proprietary. NP_002495.2.
Database link: Q12986 -
阳性对照
- HeLa, 293T and NIH3T3 whole cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176733于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
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| 说明 |
|---|
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IP
Use at 2-5 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 124 kDa. |
靶标
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相关性
NFX1 is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. It may play a role in the inflammatory response, regulating its duration by limiting the period in which class II MHC molecules are induced by IFN gamma. The RING type zinc finger domain interacts with an ubiquitin conjugating enzyme (E2) and facilitates ubiquitination. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 4799 Human
- Entrez Gene: 74164 Mouse
- Omim: 603255 Human
- SwissProt: Q12986 Human
- SwissProt: B1AY10 Mouse
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别名
- MGC20369 antibody
- NFX 1 antibody
- NFX1 antibody
see all
图片
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Western blot - Anti-NFX1 antibody (ab176733)All lanes : Anti-NFX1 antibody (ab176733) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-NFX1 antibody (ab176733)ab176733 at 0.4 µg/ml detecting NFX1 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of NFX1.
Lane 2: IP with an antibody which recognizes an upstream epitope of NFX1.Lane 3: ab176733 at 3 µg/mg of lysate
Lane 4: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.
数据表及文件
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Datasheet download
文献 (1)
ab176733 被引用在 1 文献中.
- Wu P et al. Tumor Cell-Derived TGFß1 Attenuates Antitumor Immune Activity of T Cells via Regulation of PD-1 mRNA. Cancer Immunol Res 8:1470-1484 (2020). PubMed: 32999004
