AB175192

重组Anti-NFkB p100/NFKB2抗体[EPR4686-66]

Name: NFkB p100/NFKB2抗体[EPR4686-66] (ab175192)| Abcam中文官网
Brand: Abcam Limited
SKU: AB175192
Price: 2391 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-NFkB p100/NFKB2 antibody [EPR4686-66]

RabMAb
Recombinant
KO Validated
了解详情

(1 Review)

(5 Publications)

Rabbit Recombinant Monoclonal NFkB p100/NFKB2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 5 publications.

查看别名

LYT10, NFKB2, Nuclear factor NF-kappa-B p100 subunit, DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10 protein, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, Oncogene Lyt-10, Lyt10

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)

ab175192 staining NFkB p100/NFKB2 in wild-type HAP1 cells (top panel) and NFkB p100/NFKB2 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min) permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab175192 at 1/250 dilution and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

Supplier Data

Immunoprecipitation - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)

Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate labeling NFkB p100/NFKB2 with ab175192 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (ab175192)

Predicted band size: 97 kDa

false

Lab

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)

Lanes 1- 2 : Merged signal (red and green). Green - ab175192 observed at 97 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab175192 was shown to react with NFkB p100/NFKB2 in wild-type HCT116 cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab266883 (CRISPR/Cas9 edited cell lysate ab257245) lane below 97kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HCT116 and NFKB2 CRISPR/Cas9 edited HCT117 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab175192 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (ab175192) at 1/1000 dilution

Lane 1:

Wild-type HCT116 cell lysate at 20 µg

Lane 2:

Western blot - Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell line (<a href='/products/cell-lines/human-nfkb2-nfkb-p100-nfkb2-knockout-hct116-cell-line-ab266883'>ab266883</a>)

Lane 2:

NFKB2 CRISPR/Cas9 edited HCT116 cell lysate at 20 µg

Predicted band size: 97 kDa

Observed band size: 120 kDa

false

Supplier Data

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)

All lanes:

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (ab175192) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Daudi cell lysates at 10 µg

Lane 4:

MCF7 cell lysate at 10 µg

Predicted band size: 97 kDa

false

Lab

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)

Lanes 1- 2 : Merged signal (red and green). Green - ab175192 observed at 120 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab175192 was shown to react with NFkB p100/NFKB2 in wild-type HepG2 cells in western blot. The band observed in knockout cell line ab262323 (knockout cell lysate ab257247) lane below 97kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HepG2 and NFKB2 knockout HepG2 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab175192 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (ab175192) at 1/1000 dilution

Lane 1:

Wild-type HepG2 cell lysate at 20 µg

Lane 2:

Western blot - Human NFKB2 (NFkB p100/NFKB2) knockout Hep G2 cell lysate (<a href='/products/cell-lysates/human-nfkb2-nfkb-p100-nfkb2-knockout-hep-g2-cell-lysate-ab257247'>ab257247</a>)

Predicted band size: 97 kDa

Observed band size: 120 kDa

false

Lab

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)

Lanes 1- 2 : Merged signal (red and green). Green - ab175192 observed at 120 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab175192 was shown to react with NFkB p100/NFKB2 in wild-type HCT116 cells in western blot. The band observed in knockout cell line ab266883 (knockout cell lysate ab257245) lane below 97kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HCT116 and NFKB2 knockout HCT117 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab175192 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (ab175192) at 1/1000 dilution

Lane 1:

Wild-type HCT116 cell lysate at 20 µg

Lane 2:

Western blot - Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell lysate (<a href='/products/cell-lysates/human-nfkb2-nfkb-p100-nfkb2-knockout-hct116-cell-lysate-ab257245'>ab257245</a>)

Predicted band size: 97 kDa

Observed band size: 120 kDa

false

Lab

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)

Lanes 1- 2 : Merged signal (red and green). Green - ab175192 observed at 120 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab175192 was shown to react with NFkB p100/NFKB2 in wild-type HepG2 cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab262323 (CRISPR/Cas9 edited cell lysate ab257247) lane below 97kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HepG2 and NFKB2 CRISPR/Cas9 edited HepG2 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab175192 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (ab175192) at 1/1000 dilution

Lane 1:

Wild-type HepG2 cell lysate at 20 µg

Lane 2:

Western blot - Human NFKB2 (NFkB p100/NFKB2) knockout Hep G2 cell line (<a href='/products/cell-lines/human-nfkb2-nfkb-p100-nfkb2-knockout-hep-g2-cell-line-ab262323'>ab262323</a>)

Lane 2:

NFKB2 CRISPR/Cas9 edited HepG2 cell lysate at 20 µg

Predicted band size: 97 kDa

Observed band size: 120 kDa

false

Lab

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : NFκB p100 knockout HAP1 cell lysate (20 μg)
Lane 3 : Jurkat cell lysate (20 μg)
Lane 4 : HeLa cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab175192 observed at 97 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab175192 was shown to specifically react with NFκB p100 when NFκB p100 knockout samples were used. Wild-type and NFκB p100 knockout samples were subjected to SDS-PAGE. ab175192 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (ab175192)

Predicted band size: 97 kDa

false

不同偶联物与剂型 (3)

Carrier free

Anti-NFkB p100/NFKB2 antibody [EPR4686-66] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-NFkB p100/NFKB2 antibody [EPR4686-66]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-NFkB p100/NFKB2 antibody [EPR4686-66]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4686-66

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IP, WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer.

See full target information NFKB2

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 26:3917-3936 PubMed40615576

2025

NAT10-mediated acetylation of NIK mRNA in B cells promotes IgA production.

Applications

Unspecified application

Species

Unspecified reactive species

Wan-Jun Jiang,Xin-Tao Mao,Wen-Ping Li,Nicole Jin,Yu Wang,Guiping Guan,Jin Jin,Yi-Yuan Li

Reproductive biology and endocrinology : RB&E 22:113 PubMed39210375

2024

Fance deficiency impaired DNA damage repair of prospermatogonia and altered the repair dynamics of spermatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Yin,Zhixian Zhou,Chun Fu

Anais da Academia Brasileira de Ciencias 95:e20220596 PubMed37132750

2023

Nuclear Factor-Kappa B-induced miRNA-518a-5p represses trophoblast cell migration and invasion by the Nuclear Factor-Kappa B pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Peng,Ruirui Zhang,Yumei Zhang,Chunyan Cai

Computational and mathematical methods in medicine 2022:9588740 PubMed36118831

2022

CREB1 Transcriptionally Activates LTBR to Promote the NF-B Pathway and Apoptosis in Lung Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengyun Hu,Guoping Zhou

Annals of translational medicine 9:1624 PubMed34926668

2021

Hyperglycemia-induced increasing of /circ_0008590 in NF-κB pathway is repressed by miR-1243 in human retinal microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Shao,Jiping Cai,Yong Yao,Hui Zhu

View all publications

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For licensing inquiries, please contact partnerships@abcam.com

重组Anti-NFkB p100/NFKB2抗体[EPR4686-66]

Anti-NFkB p100/NFKB2 antibody [EPR4686-66]

Immunocytochemistry/ Immunofluorescence - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)

Immunoprecipitation - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)