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Cell Biology Apoptosis Intracellular Kinases

Anti-NFkB Inducing Kinase NIK抗体(ab7204)

  • Datasheet
  • SDS
Reviews (2)Q&A (3)References (14)

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Western blot - Anti-NFkB Inducing Kinase NIK antibody (ab7204)
  • Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody (ab7204)

Key features and details

  • Rabbit polyclonal to NFkB Inducing Kinase NIK
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-NFkB Inducing Kinase NIK antibody [3H31L48] (ab203568)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-NFkB Inducing Kinase NIK抗体
    参阅全部 NFkB Inducing Kinase NIK 一抗
  • 描述

    兔多克隆抗体to NFkB Inducing Kinase NIK
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC/IF, WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to Human NFkB Inducing Kinase NIK aa 795-947.
    Database link: Q99558

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.2
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human NFkB Inducing Kinase NIK protein (ab61262)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7204于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 5 µg/ml.
WB (2)
1/2000.
说明
ICC/IF
Use a concentration of 5 µg/ml.
WB
1/2000.

靶标

  • 功能

    Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
  • 组织特异性

    Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
  • 序列相似性

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰

    Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
    Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.
  • 细胞定位

    Cytoplasm.
  • Target information above from: UniProt accession Q99558 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 9020 Human
    • Omim: 604655 Human
    • SwissProt: Q99558 Human
    • Unigene: 404183 Human
    • Unigene: 668927 Human
    • Unigene: 735695 Human
    • 别名

      • MAP3K14 antibody
      • FTDCR1B antibody
      • HS antibody
      • HsNIK antibody
      • M3K14_HUMAN antibody
      • Map3k14 antibody
      • Mitogen activated protein kinase kinase kinase 14 antibody
      • Mitogen-activated protein kinase kinase kinase 14 antibody
      • NF kappa beta inducing kinase antibody
      • NF-kappa-beta-inducing kinase antibody
      • NIK antibody
      • Serine/threonine-protein kinase NIK antibody
      see all

    图片

    • Western blot - Anti-NFkB Inducing Kinase NIK antibody (ab7204)
      Western blot - Anti-NFkB Inducing Kinase NIK antibody (ab7204)
      All lanes : Anti-NFkB Inducing Kinase NIK antibody (ab7204)

      Lane 1 : macrophages lysate
      Lane 2 : NIK transfected cell lysate


      Western blot detection of NIK in 1, macrophage and 2, NIK transfected cells by anti-human NIK.

    • Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody (ab7204)
      Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody (ab7204)
      ICC/IF image of ab7204 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (14)

    发表研究结果有使用 ab7204?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab7204 被引用在 14 文献中.

    • Afsahi A  et al. LCL161 enhances expansion and survival of engineered anti-tumor T cells but is restricted by death signaling. Front Immunol 14:1179827 (2023). PubMed: 37138866
    • Xu Z  et al. lncRNA PCGEM1 strengthens anti-inflammatory and lung protective effects of montelukast sodium in children with cough-variant asthma. Braz J Med Biol Res 53:e9271 (2020). PubMed: 32520202
    • Fang C  et al. ZFYVE21 is a complement-induced Rab5 effector that activates non-canonical NF-?B via phosphoinosotide remodeling of endosomes. Nat Commun 10:2247 (2019). PubMed: 31113953
    • Wang X  et al. Tunicamycin suppresses breast cancer cell growth and metastasis via regulation of the protein kinase B/nuclear factor-?B signaling pathway. Oncol Lett 15:4137-4142 (2018). PubMed: 29541178
    • Yu R  et al. Kruppel-like factor 4 inhibits non-small cell lung cancer cell growth and aggressiveness by stimulating transforming growth factor-ß1-meidated ERK/JNK/NF-?B signaling pathways. Tumour Biol 39:1010428317705574 (2017). PubMed: 28631556
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-NFkB Inducing Kinase NIK antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Mouse Cell lysate - whole cell (colon)
    Specification
    colon
    Treatment
    overexpression
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    XXXXXX XXXXXX

    Verified customer

    提交于 Apr 23 2014

    Western blot abreview for Anti-NFkB Inducing Kinase NIK antibody

    Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Loading amount
    20 µg
    Specification
    HEK293
    Gel Running Conditions
    Non-reduced Denaturing (10% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 16 2012

    Question

    is this a positive control for ab7204? is this good for running on a western for a postive control?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 31 2006

    Answer

    Thank you for your secondary. Ab7092 - Goat polyclonal to Rat IgG H&L Pre-Adsorbed - is a secondary antibody and is not a positive control for ab7204. Ab7204 was characterized for application in Western blotting using NIK transfected cell lysate which would be recommended as a positive control. There is an image showing this on the online datasheet. Please contact us again if you have any additional questions.

    Read More

    Abcam Scientific Support

    回复于 Jan 31 2006

    Question

    Is there new data to indicate that the human NIK antibody (ab7204) cross-reacts with mouse NIK? or, Is there a mouse NIK antibody available?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 18 2003

    Answer

    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with mouse has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. We currently do not have a mouse NIK antibody. To see is another company may have what you are looking for, I suggest that you click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249). If you have ant more questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Nov 18 2003

    Question

    Is this antibody able to detect endogenous NIK in western blot or only the transfected one? Thank you for your help

    Read More

    Abcam community

    Verified customer

    Asked on Sep 13 2001

    Answer

    We haven't tested endogenous NIK but one of our customers has got this to reliably work for detecting endogenous NIK.

    Read More

    Neil Hayward

    Abcam Scientific Support

    回复于 Sep 17 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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